Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inflammation01:38

Inflammation

Overview
Lipid-derived Compounds in the Human Body01:31

Lipid-derived Compounds in the Human Body

Fats and lipids are crucial components in the human body. Some lipid-derived compounds, such as fat-soluble vitamins, eicosanoids, lipoproteins, and glycolipids, also play unique roles to support various  biological processes .
Fat-soluble Vitamins
Fat-soluble vitamins, including vitamins A, D, E, and K, are required in minimal quantities, but their deficiencies can lead to severely abnormal physiological conditions. For example, vitamin A deficiency can cause night blindness, dry skin, delayed...
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Structure of Lipids03:38

Structure of Lipids

Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic birds and...
Atherosclerosis I: Introduction01:30

Atherosclerosis I: Introduction

Atherosclerosis is a progressive disorder characterized by the buildup of plaques on the arterial inner wall, causing them to narrow and harden over time. These plaques comprise lipids, calcium, blood components, carbohydrates, and fibrous tissue. The process primarily affects the intima of large and medium-sized arteries, reducing blood flow in any artery.Etiology and risk factorsThe cause of atherosclerosis is multifactorial, involving a complex interplay among endothelial injury, lipid...
Chronic Inflammation: Introduction01:12

Chronic Inflammation: Introduction

Chronic inflammation is a prolonged, dysregulated immune response that persists for weeks to years when the inciting stimulus is difficult to eradicate or when self‑antigens drive ongoing reactivity. Morphologically, it is defined by mononuclear cell infiltration, progressive tissue destruction, and concurrent attempts at healing via angiogenesis and fibrosis. Compared with acute inflammation, edema is less prominent while cellular infiltration predominates; triggers include persistent...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lipoprotein(a) Concentration and Cardiovascular Disease in a Group of Patients With Familial Hypercholesterolemia-A Lipid Clinic Experience.

Clinical cardiology·2025
Same author

How much of the predisposition to Hashimoto's thyroiditis can be explained based on previously reported associations?

Journal of endocrinological investigation·2018
Same author

FAMILIARITY TRANSFER AS AN EXPLANATION OF THE DÉJÀ VU EFFECT.

Psychological reports·2015
Same author

Bacterial adaptation to cold.

Microbiology (Reading, England)·2013
Same author

Vitamin D receptor gene polymorphism Fok I in the Polish population does not contribute to the risk of systemic lupus erythematosus.

Lupus·2013
Same author

Doubling the referral rate of monogenic diabetes through a nationwide information campaign--update on glucokinase gene mutations in a Polish cohort.

Clinical genetics·2011
Same journal

Danggui Shaoyao San alleviates nephrotic syndrome by regulating inerleukin 13/signal transducer and activator of transcription 6 axis.

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society·2026
Same journal

Effect of short-term of vaginal therapy with prasterone before urogynecological surgery in woman.

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society·2026
Same journal

Parthenolide ameliorates metabolic dysfunction-associated steatohepatitis by inhibiting M1 polarization by suppressing the nuclear factor-κB pathway.

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society·2026
Same journal

Glutamyl-tRNA synthetase 2 regulates mitochondrial biogenesis and reactive oxygen species homeostasis mediatingenzalutamide resistance by targeting striatin 4 in prostate cancer cells.

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society·2026
Same journal

Regulation of the KEAP1/NRF2 pathway by claudin-2-targeted lymphoid enhancer-binding factor 1 binding in gastric cancer progression.

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society·2026
Same journal

Mulberry leaf supplementation improves glycemic control but not renal or cardiovascular markers in spontaneously hypertensive rats.

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society·2026
See all related articles

Related Experiment Video

Updated: Jun 1, 2026

Quantification of Endothelial Fatty Acid Uptake using Fluorescent Fatty Acid Analogs
06:03

Quantification of Endothelial Fatty Acid Uptake using Fluorescent Fatty Acid Analogs

Published on: August 15, 2025

Trans fatty acids induce a proinflammatory response in endothelial cells through ROS-dependent nuclear factor-κB

D Bryk1, D Zapolska-Downar, M Malecki

  • 1Department of Biochemistry and Clinical Chemistry, The Warsaw Medical University, Warsaw, Poland.

Journal of Physiology and Pharmacology : an Official Journal of the Polish Physiological Society
|June 16, 2011
PubMed
Summary
This summary is machine-generated.

Trans fatty acids (TFAs) promote inflammation and leukocyte adhesion to endothelial cells. This process involves reactive oxygen species (ROS) and nuclear factor-kappa B (NF-κB) activation, contributing to vascular disease risk.

More Related Videos

Visualization of Vascular Ca2+ Signaling Triggered by Paracrine Derived ROS
07:33

Visualization of Vascular Ca2+ Signaling Triggered by Paracrine Derived ROS

Published on: December 21, 2011

Using En Face Immunofluorescence Staining to Observe Vascular Endothelial Cells Directly
06:09

Using En Face Immunofluorescence Staining to Observe Vascular Endothelial Cells Directly

Published on: August 20, 2019

Related Experiment Videos

Last Updated: Jun 1, 2026

Quantification of Endothelial Fatty Acid Uptake using Fluorescent Fatty Acid Analogs
06:03

Quantification of Endothelial Fatty Acid Uptake using Fluorescent Fatty Acid Analogs

Published on: August 15, 2025

Visualization of Vascular Ca2+ Signaling Triggered by Paracrine Derived ROS
07:33

Visualization of Vascular Ca2+ Signaling Triggered by Paracrine Derived ROS

Published on: December 21, 2011

Using En Face Immunofluorescence Staining to Observe Vascular Endothelial Cells Directly
06:09

Using En Face Immunofluorescence Staining to Observe Vascular Endothelial Cells Directly

Published on: August 20, 2019

Area of Science:

  • Cardiovascular Science
  • Molecular Biology
  • Cell Biology

Background:

  • Increased trans fatty acid (TFA) intake is linked to higher cardiovascular disease risk.
  • Endothelial cell inflammation is a critical factor in vascular disease development.

Purpose of the Study:

  • To investigate the effects of linoelaidic acid (LA) and elaidic acid (EA) on endothelial cells' proinflammatory response.
  • To elucidate the mechanisms underlying TFA-induced endothelial activation.

Main Methods:

  • Human aortic endothelial cells (HAECs) were treated with LA or EA.
  • Assessed ICAM-1 and VCAM-1 expression via flow cytometry and RT-PCR.
  • Evaluated leukocyte adhesion, NF-κB activation, and reactive oxygen species (ROS) production.

Main Results:

  • LA and EA significantly increased ICAM-1 and VCAM-1 expression, leukocyte adhesion, NF-κB phosphorylation, and ROS generation.
  • NF-κB inhibition blocked TFA-induced ICAM-1/VCAM-1 expression.
  • NADPH oxidase inhibition abolished TFA-induced ROS production and downstream effects.

Conclusions:

  • Dietary TFAs exert a direct proinflammatory effect on the endothelium.
  • Leukocyte adhesion is promoted via ROS-dependent NF-κB activation by TFAs.