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Designing label-free DNA sequences to achieve controllable turn-off/on fluorescence response for Hg2+ detection

Zece Zhu1, Liang Xu, Xiang Zhou

  • 1Department of Chemistry, Wuhan University, Wuhan 430072, China.

Chemical Communications (Cambridge, England)
|June 16, 2011
PubMed

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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...