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Related Concept Videos

Anionic Chain-Growth Polymerization: Overview01:20

Anionic Chain-Growth Polymerization: Overview

The polymerization process that involves carbanion as an intermediate is called anionic polymerization. It is also a type of addition or chain-growth polymerization. Anionic polymerization gets initiated by a strong nucleophile such as an organolithium or a Grignard reagent. The most commonly used initiator for anionic polymerization is butyl lithium. Monomers involved in anionic polymerization must possess a vinyl group bonded to one or two electron-withdrawing groups. For instance,...

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Related Experiment Video

Updated: Jun 1, 2026

Fabricating Reactive Surfaces with Brush-like and Crosslinked Films of Azlactone-Functionalized Block Co-Polymers
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Published on: June 30, 2018

Enzyme immobilization on reactive polymer films.

Ana L Cordeiro, Tilo Pompe, Katrin Salchert

    Methods in Molecular Biology (Clifton, N.J.)
    |June 16, 2011
    PubMed
    Summary

    This study presents a versatile method for immobilizing enzymes onto solid supports using maleic anhydride copolymers. This technique allows precise control over enzyme concentration and activity for bioanalytical applications.

    Area of Science:

    • Biotechnology
    • Materials Science
    • Biochemistry

    Background:

    • Immobilized enzymes are crucial for bioanalytical and biomedical applications.
    • Current methods require precise control over enzyme concentration and activity.
    • Developing versatile immobilization techniques is essential for broader applications.

    Purpose of the Study:

    • To describe a protocol for covalently attaching enzymes to solid supports using maleic anhydride copolymer thin films.
    • To demonstrate tunable control over enzyme concentration and activity.
    • To showcase the versatility of the method across various solid supports and biomolecules.

    Main Methods:

    • Utilizing thin films of maleic anhydride copolymers for enzyme immobilization.
    • Covalently attaching enzymes with primary amino groups to diverse solid supports (silicon oxide, polystyrene, metallic surfaces).

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    Published on: September 23, 2013

    Reactive Vapor Deposition of Conjugated Polymer Films on Arbitrary Substrates
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    Reactive Vapor Deposition of Conjugated Polymer Films on Arbitrary Substrates

    Published on: January 17, 2018

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    Fabricating Reactive Surfaces with Brush-like and Crosslinked Films of Azlactone-Functionalized Block Co-Polymers
    10:09

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    Published on: June 30, 2018

    Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
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    Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

    Published on: September 23, 2013

    Reactive Vapor Deposition of Conjugated Polymer Films on Arbitrary Substrates
    07:32

    Reactive Vapor Deposition of Conjugated Polymer Films on Arbitrary Substrates

    Published on: January 17, 2018

  • Tuning enzyme concentration and activity by adjusting polymer properties and enzyme loading.

    • Main Results:

    • Successful immobilization of the proteolytic enzyme Subtilisin A with tunable concentration and activity.
    • Demonstrated covalent attachment to a wide range of solid carriers.
    • Established a versatile platform for enzyme immobilization.

    Conclusions:

    • The maleic anhydride copolymer method offers a versatile and controllable approach for enzyme immobilization.
    • This technique facilitates applications in cell adhesion studies and reaction catalysis.
    • The protocol enables precise control over surface-bound enzyme characteristics for bioanalytical tools.