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Small-Scale Extraction of Caenorhabditis elegans Genomic DNA

Small-Scale Extraction of Caenorhabditis elegans Genomic DNA

Published on: June 7, 2022

Coelomic fluid: a noninvasive source of DNA in earthworms

Yukio Minamiya¹, Kyohei Ohga, Hiroshi Hayakawa

¹Faculty of Agriculture, Kochi University, Monobe, Nankoku 783-8502, Japan. b0mf0252000@yahoo.co.jp

Molecular Ecology Resources

|June 17, 2011

Abstract:

To investigate whether coelomic fluid secreted by earthworms can be a noninvasive source of DNA, we amplified and sequenced DNA extracted from the coelomic fluid and muscle tissue of eight worms. The sequences obtained using DNA extracted from both sources were identical. All cytochrome c oxidase subunit I (COI) mitochondrial DNA sequences, including those retrieved from GenBank, formed a monophyletic group of Metaphire sieboldi. The results indicate that we successfully extracted total DNA from coelomic fluid secreted by earthworm.

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