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Updated: Jun 1, 2026

RNA Interference in the Egg Parasitoid, Trichogramma dendrolimi Matsumura
Published on: November 21, 2023
Characterization of an intergenic polymorphic site (pp-hC1A_5) in Wolbachia pipientis (wPip)
Michael Petridis1, Dimitrios Chatzidimitriou
1Department of Parasitology and Infectious Diseases, Veterinary School, Aristotle University of Thessaloniki, Thessaloniki 54124, Greece. petridi@vet.auth.gr
Abstract:
Wolbachia pipientis (wPip) is an intracellular bacterium causing cytoplasmic incompatibility in arthropods, including mosquitoes of the Culex pipiens complex. Here, we present a method useful for genotyping within the wPip group. Primers were designed using a Tandem Repeat Finder program to amplify an intergenic, polymorphic site (pp-hC1A_5) of wPip. The polymorphic site is located between genes that code for polynucleotide phosphorylase and a hypothetical protein (C1A_5). Comparison of these wPip genomic regions from C. pipiens mosquitoes sampled in different geographic regions revealed deletions of fragments that proved useful in phylogenetic analysis.
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