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Determination of palonosetron in human urine by LC-MS/MS
Yuanyuan Wang1, Yongqing Wang, Peipei Zhang
1Department of Pharmacy, The First Affiliated Hospital with Nanjing Medical University, Nanjing, China. wangyy1978@gmail.com
Background:
Palonosetron is used for the prevention of chemotherapy-induced nausea and vomiting. However, quantification of this drug in human urine has been rare.
Results:
A one-step dilution method for the analysis of palonosetron in human urine using LC coupled to positive MS/MS has been developed and validated according to US FDA guidelines. The method uses 200 µl of urine and covers a working range from 2.5-1000 ng/ml with a LLOQ of 2.5 ng/ml.
Conclusion:
This new LC-MS/MS assay is sensitive and specific despite using an external standard method. It is suitable for clinical studies of palonosetron.
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