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Related Experiment Video

Updated: May 31, 2026

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
10:47

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

Published on: March 24, 2016

MALDI imaging techniques dedicated to drug-distribution studies.

David Bonnel1, Raphael Legouffe, Nicolas Willand

  • 1ImaBiotech, 59655-Villeneuve d'Ascq, France.

Bioanalysis
|June 18, 2011
PubMed
Summary

Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-MS imaging) enables simultaneous detection of thousands of compounds in tissue. This technique is ideal for tracking drugs and their metabolites in absorption, distribution, metabolism, and excretion (ADME) studies.

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Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Pharmacology

Background:

  • Mass spectrometry (MS) has evolved from analyzing molecules in solution to imaging localized biomolecules on tissue surfaces.
  • MALDI imaging MS allows simultaneous, label-free detection of thousands of compounds within a tissue section.
  • Initially used for peptides and proteins, it is now applied to drug detection, making it suitable for ADME studies.

Purpose of the Study:

  • To present the capabilities of MALDI imaging MS for studying drug and metabolite distribution.
  • To demonstrate the application of MALDI imaging MS in ADME research.
  • To showcase drug distribution studies using various MS modes.

Main Methods:

  • Utilizing MALDI imaging MS for simultaneous, label-free detection of compounds in tissue sections.
  • Applying MS, MS/MS, FAST-SRM, and MRM modes for drug and metabolite analysis.
  • Investigating drug distribution in specific tissues and whole-body sections.

Main Results:

  • MALDI imaging MS effectively tracks administered drugs and their metabolites.
  • The study presents two case examples: olanzapine distribution in the kidney and BDM31343 distribution in a mouse whole-body section.
  • Simultaneous detection of multiple compounds is achieved without the need for labeling.

Conclusions:

  • MALDI imaging MS is a powerful tool for visualizing the spatial distribution of drugs and their metabolites in biological tissues.
  • This technique significantly enhances ADME studies by providing detailed distribution information.
  • The presented methods offer comprehensive insights into drug disposition and pharmacokinetic processes.