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New approach to modulate retinal cellular toxic effects of high glucose using marine epa and dha
Mélody Dutot1, Violaine de la Tourrette, Roxane Fagon
1Yslab, Quimper, France. melody.dutot@yslab.fr.
Background:
Protective effects of omega-3 fatty acids against cellular damages of high glucose were studied on retinal pigmented epithelial (RPE) cells.
Methods:
Retinal epithelial cells were incubated with omega-3 marine oils rich in EPA and DHA and then with high glucose (25 mM) for 48 hours. Cellular responses were compared to normal glucose (5 mM): intracellular redox status, reactive oxygen species (ROS), mitochondrial succinate deshydrogenase activity, inflammatory cytokines release and caveolin-1 expression were evaluated using microplate cytometry, ELISA and flow cytometry techniques. Fatty acids incorporation in retinal cell membranes was analysed using chromatography.
Results:
Preincubation of the cells with fish oil decreased ROS overproduction, mitochondrial alterations and TNFα release. These protective effects could be attributed to an increase in caveolin-1 expression induced by marine oil.
Conclusion:
Marine formulations rich in omega-3 fatty acids represent a promising therapeutic approach for diabetic retinopathy.
