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Published on: December 10, 2021
[Systemic oxidative stress induced by intratracheal instilling with PM10 in rats]
Xiu-ling Lu1, Xin-ru Zhang, Fu-rong Deng
1Department of Occupational & Environmental Health Sciences, Peking University School of Public Health, Beijing 100191, China.
Objective:
To observe the oxidative stress of serum, lung and heart tissues in rats caused by PM10.
Methods:
In the study,24 male Wistar rats weighed 320-360 g were randomly divided into four groups, including one saline control group and three PM10 exposed groups. PM10 was administered to the exposed groups by intratracheal instillation at the doses of 3.75, 7.5, 15 mg/kg, respectively. The rats were sacrificed 24 hours after exposure. The levels of MDA and activities of SOD in the serum, lung and heart tissues were measured. In addition, the contents of protein carbonyl in lung and heart tissues were determined.
Results:
The activities of SOD in the serum, lung and heart tissues decreased markedly in the 7.5 and 15 mg/kg groups. The levels of MDA in the lung tissues significantly increased in the 7.5 and 15 mg/kg groups, while those in the serum and heart tissues significantly increased only in the 15 mg/kg group. The contents of protein carbonyl in the lung tissues markedly increased in the 15 mg/kg group, but no significant change was found in the heart tissues.
Conclusion:
The study showed that PM10 can cause oxidative stress effects in rats. The lipids of cell membrane are more susceptible to oxidative stress than the proteins.
