Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal crystal...
X-ray Crystallography02:18

X-ray Crystallography

The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
X-ray Imaging01:24

X-ray Imaging

German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with X-rays, and by 1900, X-ray was widely...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which are...
Atomic Absorption Spectroscopy: Radiation and Light Sources01:13

Atomic Absorption Spectroscopy: Radiation and Light Sources

Atomic absorption spectroscopy (AAS) relies on the Beer-Lambert law, which requires that the radiation source emits a narrow range of wavelengths to match the absorption characteristics of the analyte atom. The primary criteria for choosing an appropriate radiation source in AAS is to provide a precise and intense emission at specific wavelengths that will allow accurate detection of the analyte.
Two common narrow-range 'line' sources used in AAS are hollow-cathode lamps (HCLs) and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biomechanical evaluation of the impact of collared cementless total hip arthroplasty stems on implant subsidence: a cadaveric study in German Shepherd.

Iranian journal of veterinary research·2024
Same author

Clinical and imaging features of drug-susceptible and multidrug-resistant TB in Korean adults.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2023
Same author

Time-resolved β-lactam cleavage by L1 metallo-β-lactamase.

Nature communications·2022
Same author

Search for Subsolar-Mass Binaries in the First Half of Advanced LIGO's and Advanced Virgo's Third Observing Run.

Physical review letters·2022
Same author

Scintillation light detection in the 6-m drift-length ProtoDUNE Dual Phase liquid argon TPC.

The European physical journal. C, Particles and fields·2022
Same author

Prospects for beyond the Standard Model physics searches at the Deep Underground Neutrino Experiment: DUNE Collaboration.

The European physical journal. C, Particles and fields·2021
Same journal

Launching a new era for Short Communications in Journal of Synchrotron Radiation.

Journal of synchrotron radiation·2026
Same journal

Sagittal collimating diaboloid: a new grazing-incidence mirror surface for higher-throughput resonant inelastic X-ray scattering spectrometers.

Journal of synchrotron radiation·2026
Same journal

Synchrotron X-ray tomography and spectroscopy in numismatics: disclosing counterfeit practices in medieval silver coins.

Journal of synchrotron radiation·2026
Same journal

The Big Data Science Center at the Shanghai Synchrotron Radiation Facility: the architecture of the superfacility.

Journal of synchrotron radiation·2026
Same journal

A robotic and high-throughput X-ray micro-computed tomography workflow.

Journal of synchrotron radiation·2026
Same journal

Evolution of hierarchical phase-contrast tomography on the European Synchrotron beamlines BM05 and BM18: a whole adult human brain imaging case study.

Journal of synchrotron radiation·2026
See all related articles

Related Experiment Video

Updated: May 31, 2026

Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers
07:26

Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers

Published on: February 28, 2019

BioCARS: a synchrotron resource for time-resolved X-ray science.

T Graber1, S Anderson, H Brewer

  • 1Center for Advanced Radiation Sources, The University of Chicago, Chicago, IL 60637, USA. graber@cars.uchicago.edu

Journal of Synchrotron Radiation
|June 21, 2011
PubMed
Summary
This summary is machine-generated.

The upgraded BioCARS beamline now offers 100 ps time-resolved X-ray crystallography, enabling detailed studies of molecular dynamics. This advancement provides high-flux capabilities comparable to advanced X-ray free-electron lasers.

More Related Videos

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
08:46

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples
10:12

Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples

Published on: June 19, 2018

Related Experiment Videos

Last Updated: May 31, 2026

Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers
07:26

Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers

Published on: February 28, 2019

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
08:46

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples
10:12

Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples

Published on: June 19, 2018

Area of Science:

  • Structural Biology
  • Biophysics
  • Materials Science

Background:

  • Macromolecular time-resolved X-ray crystallography requires advanced synchrotron facilities.
  • Previous limitations in time resolution hindered the study of ultrafast molecular processes.

Purpose of the Study:

  • To detail the commissioning and capabilities of the upgraded BioCARS beamline.
  • To enable single-shot laser-pump X-ray-probe experiments with picosecond resolution.

Main Methods:

  • Utilized upgraded undulator-based X-ray source at the Advanced Photon Source (APS).
  • Implemented a high-speed chopper for single X-ray pulse isolation (1 kHz).
  • Integrated a picosecond laser system synchronized to the storage ring RF clock.

Main Results:

  • Achieved time resolution as short as 100 ps for X-ray-probe measurements.
  • High-flux pink-beam capability at 12 keV and tunable first-harmonic coverage (6.8–19 keV).
  • Focused X-ray beam spot size of 90 µm (horizontal) by 20 µm (vertical).

Conclusions:

  • The upgraded BioCARS beamline significantly enhances capabilities for time-resolved crystallography.
  • Enables investigation of rapid structural changes in macromolecules with unprecedented detail.
  • Provides a powerful tool for studying dynamic processes in various scientific fields.