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[Endophthalmitis induced by herpes simplex virus, a study on early phase]
M Yoshioka1, H Ohkuma, M Uyama
1Department of Ophthalmology, Kansai Medical University, Moriguchi, Japan.
Nippon Ganka Gakkai Zasshi
|April 1, 1990
Summary
Herpes simplex virus type-1 (HSV-1) causes necrotizing retinitis in herpetic endophthalmitis. Viral replication in the inner nuclear layer initiates retinal lesions, leading to necrosis.
Area of Science:
- Ophthalmology
- Virology
- Cell Biology
Background:
- Herpetic endophthalmitis is a severe ocular inflammation.
- Herpes simplex virus type-1 (HSV-1) is a common cause of viral eye infections.
Purpose of the Study:
- To elucidate the early pathogenesis of HSV-1-induced necrotizing retinitis.
- To determine the initial sites of viral localization within the retina.
Main Methods:
- HSV-1 Miyama strain was inoculated into Vero cells.
- Viral suspension was intravitreally injected into rabbits.
- Ocular and retinal changes were monitored clinically and histologically.
Main Results:
- Intravitreal HSV-1 inoculation induced iritis, vitreal opacities, and retinal exudates in rabbits.
- Retinal lesions originated from necrotic materials in the inner nuclear layer.
- Viral particles were initially detected in the inner nuclear layer, subsequently spreading to Müller cells, ganglion cells, and nerve fibers.
Conclusions:
- HSV-1 replication in the inner nuclear layer is the primary event in the development of necrotizing retinitis.
- Understanding viral localization is crucial for managing herpetic endophthalmitis.