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Related Experiment Videos

Ca current facilitation during postrest recovery depends on Ca entry.

L V Hryshko1, D M Bers

  • 1Division of Biomedical Sciences, University of California, Riverside 92521-0121.

The American Journal of Physiology
|September 1, 1990
PubMed
Summary
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Calcium channel recovery after rest depends on calcium entry, influencing subsequent currents. Local intracellular calcium changes slow inactivation, affecting cardiac function.

Area of Science:

  • Cardiology
  • Electrophysiology
  • Calcium Channel Function

Background:

  • Whole cell calcium current (ICa) recovery after rest is crucial for cardiac function.
  • Understanding rest-dependent changes in ICa is vital, independent of sarcoplasmic reticulum calcium release.
  • Intracellular calcium buffering plays a role in modulating ICa dynamics.

Purpose of the Study:

  • To examine whole cell Ca current (ICa) recovery after rest in rabbit ventricular myocytes.
  • To elucidate the mechanisms underlying post-rest ICa potentiation and depression.
  • To investigate the role of intracellular and extracellular calcium concentrations in ICa modulation.

Main Methods:

  • Voltage-clamped rabbit ventricular myocytes with suppressed Na and K currents.

Related Experiment Videos

  • Use of ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (10 mM) for intracellular calcium buffering.
  • Examination of ICa recovery at different holding potentials and extracellular ion substitutions (Ba, Sr).

    • Main Results:

    • Post-rest ICa potentiation (negative staircase) resulted from increased recovery from inactivation.
    • Post-rest ICa depression followed by facilitation (positive staircase) involved decreased inactivation rate.
    • ICa facilitation depended on calcium entry, graded by extracellular calcium concentration, and was independent of sarcoplasmic reticulum release.

    Conclusions:

    • Calcium entry facilitates subsequent ICa, likely via local intracellular calcium changes near the sarcolemma.
    • These local calcium dynamics progressively slow the rate of ICa inactivation.
    • The study highlights a novel mechanism of ICa modulation critical for cardiac electrophysiology.