Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 31, 2026

Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy
08:54

Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy

Published on: June 5, 2019

Individually color-coded plasmonic nanoparticles for RGB analysis.

Yue Liu1, Jian Ling, Cheng Zhi Huang

  • 1Education Ministry Key Laboratory of Luminescence and Real-Time Analysis, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.

Chemical Communications (Cambridge, England)
|June 21, 2011
PubMed
Summary

Single gold nanoparticles (AuNPs) scattering light colors were digitally coded using the Red, Green, and Blue (RGB) system. This novel RGB analysis method was then applied to study thiol binding to AuNPs.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chiral plasmonic Cu<sub>2-x</sub>S quantum dots enable chirality-dependent fluorescent recognition of tryptophan enantiomers.

Talanta·2026
Same author

Assembly of Protein-DNA Framework Nanostructures: Structurally Defining Protein-DNA Interfaces With Aptamer.

Angewandte Chemie (International ed. in English)·2026
Same author

Fluorescence Imaging Analysis of PTK7 Clustering in Situ via a Programmable DNA Network for Apoptosis Induction and Drug Resistance Reversal.

Analytical chemistry·2026
Same author

Linkage chemistry-regulated excited-state behavior and reactive oxygen species pathways in donor-acceptor covalent organic polymers.

Journal of colloid and interface science·2026
Same author

Visualizing molecular diffusion direction and processes in the solid state via dichromatic fluorescent cocrystalization transformation.

Nature communications·2026
Same author

Real-Time Tracking of Ion Migration Process at the Single-Nanoparticle Level.

Analytical chemistry·2026

Area of Science:

  • Nanotechnology
  • Spectroscopy
  • Biochemistry

Background:

  • Single gold nanoparticles (AuNPs) exhibit unique optical properties based on their size and shape.
  • Scattering of light by nanoparticles is a key phenomenon for their optical characterization.
  • Understanding nanoparticle-surface interactions is crucial for applications in sensing and diagnostics.

Purpose of the Study:

  • To develop a digital color coding system for single AuNP scattering light.
  • To apply this RGB analysis to quantify thiol binding to AuNPs.
  • To establish a novel method for studying molecular interactions at the single-nanoparticle level.

Main Methods:

  • Single gold nanoparticle (AuNP) scattering spectra were measured.
  • Scattering colors were quantified using the tricolor (Red, Green, Blue - RGB) system.

More Related Videos

Colloidal Synthesis of Nanopatch Antennas for Applications in Plasmonics and Nanophotonics
09:12

Colloidal Synthesis of Nanopatch Antennas for Applications in Plasmonics and Nanophotonics

Published on: May 28, 2016

Fabrication of Periodic Gold Nanocup Arrays Using Colloidal Lithography
08:21

Fabrication of Periodic Gold Nanocup Arrays Using Colloidal Lithography

Published on: September 2, 2017

Related Experiment Videos

Last Updated: May 31, 2026

Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy
08:54

Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy

Published on: June 5, 2019

Colloidal Synthesis of Nanopatch Antennas for Applications in Plasmonics and Nanophotonics
09:12

Colloidal Synthesis of Nanopatch Antennas for Applications in Plasmonics and Nanophotonics

Published on: May 28, 2016

Fabrication of Periodic Gold Nanocup Arrays Using Colloidal Lithography
08:21

Fabrication of Periodic Gold Nanocup Arrays Using Colloidal Lithography

Published on: September 2, 2017

  • RGB values were correlated with the binding of thiols to the AuNP surface.

    • Main Results:

    • A direct correlation was established between the scattering light color (RGB values) of single AuNPs and their interaction with thiols.
    • The RGB analysis provided a sensitive method to detect and quantify thiol binding events.
    • Variations in RGB values indicated successful and specific binding of thiols to the AuNP surface.

    Conclusions:

    • Digital coding of scattering light colors from single AuNPs using the RGB system offers a powerful new analytical approach.
    • RGB analysis of single AuNPs is a viable method for studying binding events, particularly for thiols.
    • This technique has potential for developing advanced biosensors and diagnostic tools based on nanoparticle optical properties.