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Binding of polystyrene and carbon black nanoparticles to blood serum proteins
Stefanie Fertsch-Gapp1, Manuela Semmler-Behnke, Alexander Wenk
1Comprehensive Pneumology Center, Institute of Lung Biology and Disease and Focus Network Nanoparticles and Health, Helmholtz Zentrum München- Research Center for Environmental Health, Neuherberg/Munich, Germany.
Context:
Once inhaled, nanoparticles (NP) deposit on the lung surface and have first contact with the epithelial lung lining fluid (ELF) rich in proteins, which may bind to NP.
Objective:
In this study, we investigate the parameters that influence the binding between NP and proteins.
Materials And Methods:
We used the proteins albumin, transferrin (TF), and apolipoprotein A-1 (all known as proteins from ELF) and different NP (polystyrene NP with negative, positive, and neutral surface coatings, Printex G and Printex 90) as models.
Results:
In all cases, a linear correlation of the added NP amount and the amount of bound proteins was found and was described quantitatively by binding indices. Bovine serum albumin (BSA), TF, and apo A-1 were bound to the largest extent to hydrophobic NP, which shows the extraordinary importance of the NP's surface properties.
Discussion:
The binding index indicates the relevance of primary particle size and surface properties, including hydrophobicity.
Conclusion:
Size and surface modifications of NP determine their protein binding. Our results suggest that the formation of conjugates of BSA, TF, and Apo A-1 with NP may play an important role in their translocation across the air-blood-barrier and subsequent biokinetics.
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