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Promoter of a salinity and cold stress-induced MCM6 DNA helicase from pea

Hung Quang Dang1, Ngoc Quang Tran, Renu Tuteja

  • 1International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India.

Plant Signaling & Behavior
|June 22, 2011
PubMed

Insights

The pea MCM6 gene

Area of Science:

  • Molecular Biology
  • Plant Science
  • Genetics

Background:

  • The mini-chromosome maintenance (MCM2-7) complex is crucial for DNA replication licensing and unwinding in eukaryotes.
  • Single MCM6 subunit from pea exhibits helicase and ATPase activities in vitro.
  • MCM6 transcript levels increase in pea plants under salinity and cold stress, but not other abiotic stresses.

Purpose of the Study:

  • To investigate the promoter region of the pea MCM6 gene.
  • To identify potential stress-responsive elements within the MCM6 promoter.

Main Methods:

  • Promoter analysis of the pea MCM6 gene.
  • Bioinformatic identification of cis-regulatory elements.

Main Results:

  • The promoter of the pea MCM6 gene contains stress-responsive elements.
  • These elements are likely involved in regulating MCM6 expression under abiotic stress.

Conclusions:

  • The identified stress-responsive elements in the MCM6 promoter may explain its upregulation during abiotic stress conditions.
  • This finding contributes to understanding the molecular mechanisms of plant stress tolerance.

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