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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: May 31, 2026

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

A flexible microfluidic processor for molecular biology: application to microarray sample preparation.

Yuan Li¹, Wendell Jones, Farzaneh Rasti

¹IntegenX, 5720 Stoneridge Drive, Pleasanton, CA 94588, USA.

|June 22, 2011

Summary

A novel microfluidic system offers flexible, automated processing for molecular biology applications. This programmable device, with integrated pumps and valves, performs similarly to traditional methods for mRNA amplification and RNA analysis.

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Last Updated: May 31, 2026

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High-throughput Protein Expression Generator Using a Microfluidic Platform

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

Area of Science:

Biotechnology
Molecular Biology
Microfluidics

Background:

Traditional molecular biology protocols often require significant manual manipulation and large reaction volumes.
Microfluidic systems offer potential for miniaturization and automation, but flexibility can be a challenge.

Purpose of the Study:

To present a programmable microfluidic system with integrated pumps and valves.
To demonstrate the system's flexibility using a standard mRNA amplification protocol.
To compare the performance of the microfluidic system against conventional benchtop methods.

Main Methods:

Development of a programmable microfluidic chip with onboard pumps and valves.
Implementation of a commercial mRNA amplification protocol on the microfluidic system.
Comparative analysis of microfluidic and benchtop reactions at each protocol step.
Assessment of amplified RNA quality using DNA microarrays.

Main Results:

The microfluidic system successfully processed reaction volumes from sub-microlitre to hundred microlitre.
mRNA amplification protocol was implemented with minimal modifications.
Microchip-based reactions showed comparable results to benchtop controls at each stage.
Differential microarray analyses indicated virtually indistinguishable performance between microchip- and bench-derived amplified RNAs.

Conclusions:

The described programmable microfluidic system provides a flexible and efficient platform for molecular biology protocols.
The system demonstrates comparable performance to conventional methods for mRNA amplification and RNA analysis.
This technology has the potential to streamline molecular biology workflows and reduce reagent consumption.