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Selenium supplementation prevents lipid peroxidation caused by arduous exercise in rat brain tissue
Background:
The objective of the present study is to examine how selenium supplementation affects lipid peroxidation in the brain tissue of rats subjected to acute swimming exercise.
Methods:
The study was carried out on 32 Spraque-Dawley adult male rats, which were allocated equally to four groups, namely general control (Group 1), selenium-supplemented control (0.6 mg/kg/day sodium selenite) (Group 2), swimming control (Group 3), and selenium-supplemented swimming group (6 mg/kg/day sodium selenite) (Group 4). Selenium supplementations were carried out by intraperitoneal (i.p.) route for four weeks. At the end of supplementation, animals in groups 3 and 4 were subjected to acute swimming. Brain tissue samples were collected from decapitated animals to determine malondialdehyde (MDA) levels using a spectrophotometer according to "Schimadzu-1601, Japan" Uchiyama and Mihara and GSH levels using a spectrophotometer according to Ellmann.
Results:
The highest MDA values in the brain tissue were obtained in Group 3 (p < 0.001). MDA values in Group 4 were higher than those in Groups 1 and 2 (p < 0.001). Brain tissue MDA values in Groups 1 and 2 did not differ. Group 4 had the highest brain GSH levels (p < 0.001). Brain tissue glutathione (GSH) levels in Group 3 were higher than those in Groups 1 and 2 (p < 0.001).
Conclusion:
Results of the study indicate that acute swimming exercise in rats increased the lipid peroxidation in the brain tissue of rats, while selenium supplementation prevented the free-radical formation by enhancing the antioxidant activity (Tab. 1, Ref. 24).
