Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 31, 2026

Liver Cold Storage and Transplantation in the Cold-Adaptive Daurian Ground Squirrels

Liver Cold Storage and Transplantation in the Cold-Adaptive Daurian Ground Squirrels

Published on: July 3, 2025

Hepatocyte viability and adenosine triphosphate content decrease linearly over time during conventional cold storage

T A Berendsen¹, M L Izamis, H Xu

¹Center for Engineering in Medicine, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, and Shriners Burns Hospital, Boston, Massachusetts 02114, USA.

Transplantation Proceedings

|June 23, 2011

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Liver defatting: an alternative approach to enable steatotic liver transplantation.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2012

Same author

Isolation and culture of human endothelial cells.

Methods in molecular medicine·2011

Same author

Isolation and long-term maintenance of adult rat hepatocytes in culture.

Methods in molecular medicine·2011

Same author

Diluted blood reperfusion as a model for transplantation of ischemic rat livers: alanine aminotransferase is a direct indicator of viability.

Transplantation proceedings·2010

Same author

Selective targeting of pigmented retinal pigment epithelial (RPE) cells by a single pulsed laser irradiation: an in vitro study.

Optics express·2008

Same author

Sequential cold storage and normothermic perfusion of the ischemic rat liver.

Transplantation proceedings·2008

Static cold storage (SCS) of donor organs has limits. This study shows a linear correlation between adenosine triphosphate (ATP) levels, hepatocyte viability, and storage time, confirming ATP as a predictor of organ quality.

Area of Science:

Transplantation science
Organ preservation research
Cellular metabolism

Background:

Static cold storage (SCS) using University of Wisconsin (UW) solution is the standard for organ preservation.
The finite limits of SCS and the link between adenosine triphosphate (ATP) levels and post-preservation organ function are known, but a quantitative relationship is lacking.
Establishing this relationship is crucial for understanding preservation limits, minimizing cold ischemic injury, and maximizing donor organ utilization.

Purpose of the Study:

To determine the time limits of cellular viability and metabolic function during SCS.
To characterize the relationship between cellular viability and energetic state in organ preservation.
To utilize clinically relevant techniques for assessing organ viability.

Main Methods:

More Related Videos

Technique of Subnormothermic Ex Vivo Liver Perfusion for the Storage, Assessment, and Repair of Marginal Liver Grafts

Technique of Subnormothermic Ex Vivo Liver Perfusion for the Storage, Assessment, and Repair of Marginal Liver Grafts

Published on: August 13, 2014

Related Experiment Videos

Last Updated: May 31, 2026

Liver Cold Storage and Transplantation in the Cold-Adaptive Daurian Ground Squirrels

Liver Cold Storage and Transplantation in the Cold-Adaptive Daurian Ground Squirrels

Published on: July 3, 2025

Technique of Subnormothermic Ex Vivo Liver Perfusion for the Storage, Assessment, and Repair of Marginal Liver Grafts

Technique of Subnormothermic Ex Vivo Liver Perfusion for the Storage, Assessment, and Repair of Marginal Liver Grafts

Published on: August 13, 2014

Rat livers were procured and preserved using conventional static cold storage in UW solution at 4°C.
Hepatocyte viability and intracellular ATP content were measured at 0, 24, 48, 72, and 120 hours of storage.
Statistical analysis (ANOVA) was employed to establish relationships between parameters and storage time.

Main Results:

A steady decrease in viable hepatocytes was observed during SCS, from 25.95 × 10^6 to 0.87 × 10^6 cells/gram tissue after 5 days.
Intracellular ATP content significantly declined from 9.63 to 0.93 moles/g tissue over the same period.
A statistically significant linear correlation (P < .05) was found between both hepatocyte viability and ATP content as a function of storage time.

Conclusions:

The linear correlation between hepatocyte viability, ATP content, and storage duration suggests an underlying shared physiological basis.
Adenosine triphosphate (ATP) is confirmed as a direct predictor of organ quality in liver preservation.
These findings support quantitative assessment of donor organs, potentially improving utilization for transplantation and other applications.