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Related Experiment Video

Updated: May 31, 2026

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

Published on: June 9, 2011

ampliPHOX colorimetric detection on a DNA microarray for influenza.

Kevin R Moulton¹, Amber W Taylor, Kathy L Rowlen

¹InDevR, Inc.

Journal of Visualized Experiments : Jove

|June 23, 2011

Summary

This study introduces ampliPHOX colorimetric detection for DNA microarrays, offering a low-cost, portable alternative to fluorescence for pathogen detection, such as influenza viruses. This method provides a rapid, yes/no result for sequence presence on low-density arrays.

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Last Updated: May 31, 2026

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

Published on: June 9, 2011

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Published on: February 4, 2012

Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes

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Published on: July 26, 2019

Area of Science:

Molecular Biology
Biotechnology
Medical Diagnostics

Background:

DNA microarrays are crucial for pathogen detection, particularly for influenza virus identification and subtyping.
Current fluorescence detection methods for microarrays are effective but require expensive instrumentation.
There is a need for cost-effective, portable detection technologies for clinical and public health applications.

Purpose of the Study:

To present ampliPHOX colorimetric detection technology as a low-cost, portable alternative to fluorescence detection for DNA microarrays.
To demonstrate the application of ampliPHOX detection for identifying and characterizing influenza viruses using the FluChip microarray.
To evaluate the performance and advantages of ampliPHOX for low-density microarray applications.

Main Methods:

Developed ampliPHOX colorimetric detection for low-density DNA microarrays (≤1800 spots/array).
Utilized biotinylated targets captured on the array, followed by labeling with streptavidin-conjugate (ampliTAG).
Employed ampliPHOX Reader for polymerization (ampliPHY) upon light exposure, followed by staining and analysis with ampliVIEW software.

Main Results:

AmpliPHOX detection offers a limit of detection within one order of magnitude of fluorescence.
The ampliPHOX instrument is approximately ten-fold cheaper and more portable than fluorescence scanners.
The entire FluChip assay, including ampliPHOX detection, can be completed in approximately 6 hours, with detection taking about 30 minutes.

Conclusions:

AmpliPHOX colorimetric detection is a viable, cost-effective, and portable signal amplification method for low-density DNA microarrays.
This technology is well-suited for pathogen detection arrays requiring a yes/no result, such as influenza virus detection.
AmpliPHOX technology enhances the accessibility and cost-effectiveness of microarray-based diagnostics for public health and clinical use.