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Published on: March 29, 2018
[Effect of subchronic fluoride exposure on pathologic change and beta-catenin expression in rat bone tissue]
Xiaoying Guo1, Sisi Wu, Yang He
1Department of Biogeochemical Disease, Liaoning Provincial Key Laboratory of Arsenic Biological Effect, China Medical University, Shenyang, China. xyguo@mail.com.cn
Objective:
To explore the effect of subchronic fluoride exposure on the expression of beta-catenin in the bone of rats and the role of beta-catenin in skeletal damage.
Methods:
Wistar rats were randomly divided into four groups. The rats were treated at the doses of 50, 100 and 150 mg/L F- in drinking water, and control rats were drunk tap water. The contents of fluoride in bone and serum were determined after three month. The expressions of beta-catenin were analyzed by real-time RT-PCR and immunohistochemistry.
Results:
HE staining indicated that the compact bone was thicker and the trabecular bone increased in fluoride-treated group in comparison with control group. The hypertrophic chondrocytes accumulated and arranged disordered in fluoride-treated rats. The expressions of p-catenin mRNA and protein expressions in bones of fluoride-treated rats were higher than those of control group. Immunohistochemistry indicated that beta-catenins were mainly expressed by osteoblast and osteoclast. There was also positive staining in hypertrophic chondrocyte.
Conclusion:
It was suggested that beta-catenin could play important role in bone sclerosis of subchronic fluoride exposure.
