Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

The Bronchial Tree

The Bronchial Tree

The human bronchi and bronchial tree play a crucial role in the respiratory system, facilitating the exchange of oxygen and carbon dioxide. Let's delve into the intricate structure and functions of these respiratory components.
The trachea, commonly known as the windpipe, is a tube that connects the larynx (voice box) to the bronchi. At a point called the carina, it bifurcates into two primary bronchi. The right primary bronchus is wider, shorter, and more vertical than the left primary...

Chronic Obstructive Pulmonary Disease III: Chronic Bronchitis Features

Chronic Obstructive Pulmonary Disease III: Chronic Bronchitis Features

Chronic bronchitis is a key phenotype of chronic obstructive pulmonary disease (COPD), characterized by airway-centered inflammation and mucus overproduction. It develops from long-term exposure to harmful particles or gases, most commonly cigarette smoke, which triggers a persistent inflammatory response.Cellular and Structural ChangesInflammation initially affects the large bronchi and later the smaller airways, with infiltration by immune cells, including neutrophils, macrophages, and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Subcutaneous pedicle subunit flap for upper lip reconstruction.

JPRAS open·2025

Same author

Opportunities for nurses to address employee voice in health care providers: a scoping review.

BMC nursing·2024

Same author

Genetic screening of South African families with Parkinson's disease.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2024

Same author

Cost-Effectiveness of Prevention for People at Risk for Dementia: A Scoping Review and Qualitative Synthesis.

The journal of prevention of Alzheimer's disease·2024

Same author

Are you better than me? Social comparisons in carrion crows (Corvus corone).

Animal cognition·2023

Same author

Analgesic Medication in Fibromyalgia Patients: A Cross-Sectional Study.

Pain research & management·2022

Search research articles

Related Experiment Video

Updated: May 31, 2026

Murine Model of Allergen Induced Asthma

Murine Model of Allergen Induced Asthma

Published on: May 14, 2012

Bronchoconstriction in nonhuman primates: a species comparison.

S Seehase¹, M Schlepütz, S Switalla

¹Department of Airway Immunology, Fraunhofer Institute for Toxicology and Experimental Medicine, Hannover, Germany.

Journal of Applied Physiology (Bethesda, Md. : 1985)

|June 25, 2011

Summary

Nonhuman primate precision-cut lung slices (PCLS) offer a valuable ex vivo model for studying bronchoconstriction in respiratory diseases. Certain nonhuman primate species closely mimic human airway responses to various constrictor agents.

More Related Videos

The Bovine Lung in Biomedical Research: Visually Guided Bronchoscopy, Intrabronchial Inoculation and In Vivo Sampling Techniques

The Bovine Lung in Biomedical Research: Visually Guided Bronchoscopy, Intrabronchial Inoculation and In Vivo Sampling Techniques

Published on: July 3, 2014

Related Experiment Videos

Last Updated: May 31, 2026

Murine Model of Allergen Induced Asthma

Murine Model of Allergen Induced Asthma

Published on: May 14, 2012

The Bovine Lung in Biomedical Research: Visually Guided Bronchoscopy, Intrabronchial Inoculation and In Vivo Sampling Techniques

The Bovine Lung in Biomedical Research: Visually Guided Bronchoscopy, Intrabronchial Inoculation and In Vivo Sampling Techniques

Published on: July 3, 2014

Area of Science:

Respiratory physiology
Pharmacology
Comparative medicine

Background:

Bronchoconstriction is a key symptom in chronic obstructive respiratory diseases like asthma and COPD.
Precision-cut lung slices (PCLS) are an established ex vivo model for studying airway physiology.
Developing nonhuman primate (NHP) PCLS models is crucial for understanding species-specific responses.

Purpose of the Study:

To establish and validate an ex vivo model of bronchoconstriction using PCLS from various NHP species.
To compare the responsiveness of NHP PCLS to a range of bronchoconstrictor agents against known data from rodents, guinea pigs, and humans.

Main Methods:

PCLS were prepared from common marmosets, cynomolgus macaques, rhesus macaques, and anubis baboons.
PCLS were stimulated with increasing concentrations of methacholine, histamine, serotonin, leukotriene D₄ (LTD₄), U46619, and endothelin-1.
Airway caliber changes were measured and compared across species and with existing data.

Main Results:

Methacholine caused maximal constriction (74–88%) in all NHP species; serotonin was ineffective.
Histamine induced significant constriction (53–90%) across species, with marmosets showing lower response.
LTD₄ was effective only in cynomolgus macaques and baboons (44–49%); U46619 and endothelin-1 caused significant constriction (65–91% and 70–81%, respectively) in all NHP species.

Conclusions:

NHP PCLS provide a robust ex vivo model for investigating bronchoconstriction.
NHPs respond to key human airway mediators (methacholine, histamine, U46619, endothelin-1) but not serotonin.
Cynomolgus macaques and baboons PCLS most closely resemble human airway responses, particularly to leukotrienes.