Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Separation estimation of two freely rotating dipole emitters near the quantum limit.

Physical review. A·2026

Same author

Reconstitution strategies for exploring interactions of Ebola virus matrix protein with host membrane mimetics.

Methods in enzymology·2026

Same author

Tetraspanin CD82 shapes EGFR signaling outcomes through nanoscale receptor organization.

The Journal of cell biology·2026

Same author

IgE occupancy and antigen valency cooperate to control FcεRI aggregation geometry and signaling efficiency.

bioRxiv : the preprint server for biology·2025

Same author

Detection of PIP<sub>2</sub> distributions in biological membranes using a peptide-based sensor.

The Journal of biological chemistry·2025

Same author

Mathematical models for the EP2 and EP4 signaling pathways and their crosstalk.

Journal of theoretical biology·2025

Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 31, 2026

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

Determining FcεRI diffusional dynamics via single quantum dot tracking.

Diane S Lidke¹, Shalini T Low-Nam, Patrick J Cutler

¹Department of Pathology and Cancer Research and Treatment Center, University of New Mexico, Albuquerque, NM, USA. dlidke@salud.unm.edu

Methods in Molecular Biology (Clifton, N.J.)

|June 25, 2011

Summary

This summary is machine-generated.

Single-particle tracking with quantum dots (QDs) reveals receptor dynamics in living cells. This study details methods for QD labeling, tracking, and data analysis for IgE receptors.

More Related Videos

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)

Published on: May 27, 2012

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

Published on: February 7, 2021

Related Experiment Videos

Last Updated: May 31, 2026

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)

Published on: May 27, 2012

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

Published on: February 7, 2021

Area of Science:

Biophysics
Cell Biology
Nanotechnology

Background:

Traditional biochemical methods lack spatial-temporal resolution for receptor dynamics.
Fluorescent quantum dots (QDs) offer high brightness and photostability for live-cell imaging.
High-affinity IgE receptor dynamics are crucial for cellular signaling.

Purpose of the Study:

To establish protocols for using quantum dots (QDs) for single-particle tracking (SPT) of receptors.
To characterize the dynamics of the high-affinity IgE receptor using QD-labeled IgE.
To provide a comprehensive guide for QD conjugation, receptor tracking, and data analysis.

Main Methods:

Coupling of QDs to immunoglobulin E (IgE) antibodies.
Single-particle tracking (SPT) of individual QD-bound receptors on living cells.
Analysis of one- and two-color fluorescence tracking data.

Main Results:

Demonstrated successful conjugation of QDs to IgE.
Enabled high-resolution spatial-temporal tracking of QD-bound receptors.
Provided methods for quantitative analysis of receptor movement and interactions.

Conclusions:

Quantum dots are effective probes for single-particle tracking of cell surface receptors.
QD-based SPT offers superior resolution for studying receptor dynamics compared to biochemical methods.
The described protocols facilitate advanced investigations into receptor behavior and cellular processes.