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Updated: May 31, 2026
Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
Published on: October 15, 2013
[Analysis of an outbreak of Burkholderia cepacia for risk stratification]
Fidencia Duarte-Raya1, Martha Patricia Granados-Ramírez
1Hospital de Ginecopediatría 48, Instituto Mexicano del Seguro Social, León, Guanajuato. fidencia.duarte@imss.gob.mx
Background:
The possibility of nosocomial infection is determined by factors relating to the patient, the presence and degree of immunodeficiency, and interventions that become stronger the risk. A nosocomial outbreak is the emergence of an unusual case of a specific disease and is due to an infectious agent only. Our objective was to analyze the risk of Burkholderia cepacia with a risk stratification criterion in a pediatric intensive care unit (ICU).
Methods:
An epidemiological outbreak, transverse, retrospective study was done. We included 71 patients from a pediatric ICU. Attack rate and fatality rate was obtained. We applied Fisher exact test with a level of significance p<0.05.
Results:
Regardless the risk stratification the Burkholderia cepacia infection showed no significant difference. The attack rate was 23.94, and the fatality rate in infected patient was 29.41. Candida albicans coinfection was present in 50% of the cases; other kind of bacteria infection in 50% was also seen. Therefore the treatment was the combination of different antimicrobials.
Conclusions:
The paediatric intensive care patient is exposed to nosocomial infection including Burkholderia cepacia independent of its risk rating. Attachment and the monitoring of standard precautions with isolation strategy is suggested as a good preventive measure.
