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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: May 31, 2026

Adaptation of Semiautomated Circulating Tumor Cell (CTC) Assays for Clinical and Preclinical Research Applications
14:14

Adaptation of Semiautomated Circulating Tumor Cell (CTC) Assays for Clinical and Preclinical Research Applications

Published on: February 28, 2014

Image cytometry analysis of circulating tumor cells.

Lori E Lowes1, David Goodale, Michael Keeney

  • 1London Regional Cancer Program, London Health Sciences Centre, London, Ontario, Canada.

Methods in Cell Biology
|June 28, 2011
PubMed
Summary

Detecting circulating tumor cells (CTCs) in the blood is crucial for understanding cancer metastasis. Analyzing these rare cells offers insights into disease progression and aids in developing new cancer therapies.

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Last Updated: May 31, 2026

Adaptation of Semiautomated Circulating Tumor Cell (CTC) Assays for Clinical and Preclinical Research Applications
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Published on: February 28, 2014

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Circulating Tumor Cell Lines: an Innovative Tool for Fundamental and Translational Research
07:47

Circulating Tumor Cell Lines: an Innovative Tool for Fundamental and Translational Research

Published on: December 25, 2021

Area of Science:

  • Oncology
  • Cell Biology
  • Medical Diagnostics

Background:

  • Metastatic disease accounts for most cancer deaths.
  • Circulating tumor cells (CTCs) in the bloodstream are linked to metastasis.
  • Accurate enumeration and characterization of CTCs are vital for cancer research and patient care.

Purpose of the Study:

  • To review techniques for enriching and detecting rare circulating tumor cells (CTCs).
  • To examine the advantages and disadvantages of various CTC detection methods.
  • To provide a detailed analysis of two image cytometry approaches for CTC analysis.

Main Methods:

  • Review of existing literature on CTC detection techniques.
  • Comprehensive examination of the iCys Laser Scanning Cytometer.
  • Detailed analysis of the CellSearch® system for CTC detection.

Main Results:

  • Multiple techniques exist for CTC enrichment and detection, each with limitations.
  • Image cytometry offers valuable approaches for CTC analysis.
  • The iCys and CellSearch® systems are utilized for routine CTC analysis.

Conclusions:

  • Detecting, enumerating, and characterizing CTCs are essential tools in cancer research.
  • CTCs can illuminate the biology of the metastatic cascade.
  • Improved CTC analysis may lead to changes in patient treatment guidelines and anticancer drug development.