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Related Experiment Video

Updated: May 31, 2026

Identification of a Murine Erythroblast Subpopulation Enriched in Enucleating Events by Multi-spectral Imaging Flow Cytometry
09:40

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Published on: June 6, 2014

Background-free cytometry using rare earth complex bioprobes.

Dayong Jin1

  • 1Advanced Cytometry Labs, MQ Photonics Centre, Faculty of Science, Macquarie University, Sydney, Australia.

Methods in Cell Biology
|June 28, 2011
PubMed
Summary
This summary is machine-generated.

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Detecting rare cells, like pathogens, is challenging due to background noise. This study introduces rare-earth probes and time-gated detection for accurate, low-cost cell quantification in microbiology and diagnostics.

Area of Science:

  • Analytical microbiology
  • Disease diagnosis
  • Biotechnology
  • Nanotechnology

Background:

  • Conventional fluorescent probes struggle with rare cell quantification due to overlapping autofluorescence from background cells.
  • The 'needle-in-a-haystack' problem is severe when target cells are less than 1 in 100,000.
  • Existing methods are often expensive and lack the sensitivity for low-frequency target detection.

Purpose of the Study:

  • To present a low-cost detection technology for rapid and inexpensive quantification of rare cells.
  • To overcome spectral overlap issues caused by background autofluorescence in cell analysis.
  • To demonstrate a novel approach for sensitive detection of microbial pathogens in complex samples.

Main Methods:

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Last Updated: May 31, 2026

Identification of a Murine Erythroblast Subpopulation Enriched in Enucleating Events by Multi-spectral Imaging Flow Cytometry
09:40

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Published on: June 6, 2014

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  • Utilized rare-earth (lanthanide) complex bioprobes with long luminescence lifetimes (hundreds of microseconds).
  • Employed time-gated detection to suppress short-lived nanosecond autofluorescence from background cells.
  • Applied the technology to cytometry and imaging, including monitoring rare pathogens like Cryptosporidium parvum and Giardia lamblia.

    • Main Results:

    • Achieved sensitive detection of rare cells by minimizing background interference through time-gated detection.
    • Demonstrated effective monitoring of Cryptosporidium parvum and Giardia lamblia in concentrated drinking water samples.
    • Highlighted advancements in rare-earth nanoparticle bioprobe production for enhanced detection capabilities.

    Conclusions:

    • Rare-earth bioprobes combined with time-gated detection offer a robust solution for rare cell quantification.
    • This technology significantly improves detection sensitivity and reduces costs in microbiology and diagnostics.
    • The approach is versatile, applicable to various detection technologies including flow cytometry and imaging.