Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Raman Spectroscopy: Overview

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...

Raman Spectroscopy Instrumentation: Overview

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Tailoring the Relational Playbook for Cardiology Teams to the Rural Hospital Medicine Setting: A Multi-Method Pre-Implementation Evaluation.

The Journal of rural health : official journal of the American Rural Health Association and the National Rural Health Care Association·2026

Same author

Molecular and Structural Characterization Reveals Divergent Extracellular Vesicle Profiles Between Wild Type and Alzheimer's Disease Cerebrocortical Organoids.

bioRxiv : the preprint server for biology·2026

Same author

Acoustic separation and isolation of viruses, small extracellular vesicles and other nanoscale bioparticles.

Nature protocols·2026

Same author

Senescent cells secrete chromatin components via senescence-associated extracellular particles.

bioRxiv : the preprint server for biology·2025

Same author

Pragmatic Comparative Effectiveness of Primary Care Treatments for Posttraumatic Stress Disorder: A Randomized Clinical Trial.

JAMA psychiatry·2025

Same author

Miniaturized Workflow for Transcriptomic Profiling of Urinary Extracellular RNA during Pregnancy.

bioRxiv : the preprint server for biology·2025

Same journal

Quantification of cell viability by automated analysis of live cell imaging.

Methods in cell biology·2026

Same journal

Flow cytometry evaluation of cytotoxicity exerted by effector immune cells against tumor cells.

Methods in cell biology·2026

Same journal

Time-lapse confocal laser scanning microscopy analysis of FOOD formation.

Methods in cell biology·2026

Same journal

Screening and identification of protein-protein interaction using proximity labeling.

Methods in cell biology·2026

Same journal

Quantitative high-content profiling of mitochondrial morphology with automated statistical analysis and integrated data visualization.

Methods in cell biology·2026

Same journal

Super-resolution imaging of cell death in Drosophila tissues via expansion and pan-expansion microscopy.

Methods in cell biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 31, 2026

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

Surface-enhanced Raman scattering (SERS) cytometry.

John P Nolan¹, David S Sebba

¹La Jolla Bioengineering Institute, La Jolla, California, USA.

Methods in Cell Biology

|June 28, 2011

Summary

This summary is machine-generated.

Surface-enhanced Raman scattering (SERS) materials offer bright signals for biomolecular analysis. SERS tags with narrow spectral peaks can increase the number of measurable parameters in cytometry.

More Related Videos

Label-Free Surface-Enhanced Raman Scattering Bioanalysis Based on Au@Carbon Dot Nanoprobes

Label-Free Surface-Enhanced Raman Scattering Bioanalysis Based on Au@Carbon Dot Nanoprobes

Published on: June 9, 2023

Exploring the Application of Surface-enhanced Raman Scattering-based Biosensing of Individual sEVs in Disease Diagnosis and Therapeutics

Exploring the Application of Surface-enhanced Raman Scattering-based Biosensing of Individual sEVs in Disease Diagnosis and Therapeutics

Published on: March 13, 2026

Related Experiment Videos

Last Updated: May 31, 2026

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

Label-Free Surface-Enhanced Raman Scattering Bioanalysis Based on Au@Carbon Dot Nanoprobes

Label-Free Surface-Enhanced Raman Scattering Bioanalysis Based on Au@Carbon Dot Nanoprobes

Published on: June 9, 2023

Exploring the Application of Surface-enhanced Raman Scattering-based Biosensing of Individual sEVs in Disease Diagnosis and Therapeutics

Exploring the Application of Surface-enhanced Raman Scattering-based Biosensing of Individual sEVs in Disease Diagnosis and Therapeutics

Published on: March 13, 2026

Area of Science:

Biomolecular analysis
Spectroscopy
Materials science

Background:

Surface-enhanced Raman scattering (SERS)-active materials show promise for biomolecular analysis.
SERS offers advantages like bright signals and photostability for cytometric applications.
Current SERS cytometry is underdeveloped, requiring instrumentation and reagent advancements.

Purpose of the Study:

To address challenges in expanding fluorescent labels for cytometry.
To introduce SERS tags with narrow spectral peaks.
To enhance the efficiency of optical spectrum usage and increase cytometry parameters.

Main Methods:

Preparation and application of SERS-active materials.
Development of SERS tags with extremely narrow spectral peaks.
Exploration of SERS for cytometric analyses.

Main Results:

SERS-active materials provide bright signals, photostability, and narrow spectral features.
SERS tags with narrow spectral peaks enable more efficient use of the optical spectrum.
Potential to increase the number of measurable parameters in cytometry.

Conclusions:

SERS-active materials are advantageous for biomolecular analysis and cytometry.
SERS tags with narrow spectral peaks offer a strategy to overcome limitations in current cytometry.
Further advancements in instrumentation and reagents are crucial for SERS cytometry's potential.