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H-2 polymorphisms in outbred strains of mice
1Department of Genetics, Central Institute for Experimental Animals, Kawasaki, Japan.
Laboratory Animal Science
|September 1, 1990
Summary
We analyzed H-2 polymorphisms in five mouse strains using alloantisera. This research identified unique H-2 antigens, crucial for distinguishing between mouse strains and monitoring outbred populations.
Area of Science:
- Immunogenetics
- Mouse genetics
- Histocompatibility antigens
Background:
- The H-2 locus, the major histocompatibility complex in mice, plays a critical role in immune responses.
- Understanding H-2 polymorphisms is essential for characterizing inbred and outbred mouse strains used in research.
Purpose of the Study:
- To investigate the H-2 class I private antigen polymorphisms in five commonly used outbred mouse strains: CF1, ddY, ddN, ICR, and KUNM.
- To determine the utility of H-2 locus polymorphisms for strain characterization and monitoring.
Main Methods:
- Utilized 15 alloantisera specific for H-2 class I private antigens.
- Analyzed H-2 antigen profiles and frequencies across the five outbred mouse strains.
Main Results:
- All studied individuals expressed at least one private antigen at H-2K and/or H-2D.
- Significant variation in the number of H-2 class I private antigens and H-2 phenotypes was observed among strains.
- Strain-specific antigens were identified, enabling discrimination between CF1, ddY, ddN, ICR, and KUNM.
- Specific H-2 haplotypes (e.g., H-2k, H-2Dd, H-2Df, H-2q) and their associated antigens were found at varying frequencies within different strains.
Conclusions:
- The H-2 locus exhibits substantial polymorphism across the studied outbred mouse strains.
- H-2 antigen profiling is a valuable tool for accurately characterizing and differentiating mouse strains.
- Monitoring H-2 gene frequencies can aid in the quality control and genetic monitoring of outbred mouse populations.