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Related Concept Videos

Mitochondrial Membranes01:45

Mitochondrial Membranes

A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
Mitochondrial Membranes01:45

Mitochondrial Membranes

A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
The Inner Mitochondrial Membrane01:28

The Inner Mitochondrial Membrane

The inner mitochondrial membrane is the primary site of ATP synthesis. The inner membrane domain that forms a smooth layer adjacent to the outer membrane is called the inner boundary membrane. This domain contains membrane transporters that drive metabolites in and out of the mitochondria.  In contrast, the inner membrane network that invaginates into the matrix space is called the cristae membrane. This domain accounts for principle mitochondrial function as it accommodates the protein...
Mitochondria01:37

Mitochondria

Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
Mitochondria01:37

Mitochondria

Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
Structure of Porins01:21

Structure of Porins

Mitochondria, chloroplasts, and gram-negative bacteria have transmembrane, beta-barrel proteins called porins to mediate the free diffusion of ions and metabolites across the membrane. Mitochondrial porin precursors contain conserved amino acid sequences called beta signals at their C-terminal. Beta signals have a  motif of PoXGXXHyXHy (Po-Polar, X-Any amino acid, G-Glycine, Hy-LargeHydrophobic), which are crucial for precursor recognition to initiate precursor assembly. Beta-barrel precursors...

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Related Experiment Video

Updated: May 31, 2026

Robust Mitochondrial Isolation from Rodent Cardiac Tissue
07:03

Robust Mitochondrial Isolation from Rodent Cardiac Tissue

Published on: August 23, 2024

Mitochondria: isolation, structure and function.

Martin Picard1, Tanja Taivassalo, Gilles Gouspillou

  • 1Department of Kinesiology and Physical Education, McGill University, Montreal, Qc, Canada.

The Journal of Physiology
|June 29, 2011
PubMed
Summary
This summary is machine-generated.

Mitochondrial isolation from skeletal muscle can fragment mitochondria, altering function. Studying mitochondria in less disruptive ways is crucial for understanding their true in vivo behavior and stress resistance.

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Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue

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Last Updated: May 31, 2026

Robust Mitochondrial Isolation from Rodent Cardiac Tissue
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Published on: August 23, 2024

Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration
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Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration

Published on: September 6, 2014

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue
09:27

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue

Published on: March 23, 2015

Area of Science:

  • Cell Biology
  • Mitochondrial Biology
  • Skeletal Muscle Physiology

Background:

  • Mitochondria are dynamic organelles involved in fusion and fission, impacting morphology and function.
  • Assessing skeletal muscle mitochondrial function often involves disruptive isolation methods.
  • Current methods can alter mitochondrial structure, potentially confounding functional assessments.

Purpose of the Study:

  • To review considerations and hypotheses regarding mitochondrial function assessment in skeletal muscle.
  • To highlight the impact of isolation procedures on mitochondrial morphology and function.
  • To emphasize the value of minimally disruptive experimental preparations for studying mitochondria.

Main Methods:

  • Discussion of traditional mitochondrial isolation techniques.
  • Exploration of permeabilized sarcolemma methods preserving mitochondrial morphology.
  • Analysis of recent evidence on morphology changes and functional alterations in isolated mitochondria.

Main Results:

  • Routine mitochondrial isolation fragments skeletal muscle mitochondria, mimicking in vivo fission.
  • Fragmented mitochondrial morphology alters key functional indices.
  • Isolated mitochondria may offer insights into stress resistance, despite morphological changes.

Conclusions:

  • Caution is warranted when interpreting data from isolated skeletal muscle mitochondria.
  • Minimally disruptive preparations are valuable for studying mitochondrial function in situ.
  • Further research is needed to understand the implications of mitochondrial morphology on function.