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Updated: May 31, 2026
Loop-mediated Isothermal Amplification (LAMP) Assays for the Species-specific Detection of Eimeria that Infect Chickens
Published on: February 20, 2015
Molecular diagnosis of Eimeria species affecting naturally infected Gallus gallus
F S Carvalho1, A A Wenceslau, M Teixeira
1Programa de Pós-Graduação em Ciência Animal, Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz, Campus Universitário, Ilhéus, BA, Brasil.
Abstract:
We used PCR to test various protocols and define a technique for DNA extraction directly from chicken-shed stool samples for the identification of Eimeria species that parasitize birds. It was possible to extract and amplify DNA of seven Eimeria species from field stool samples, using both protocols tested; extractions made with phenol/chloroform protocols gave the best results. The primers were specific and sensitive, allowing amplification of samples containing as few as 20 oocysts, both in individual and in a multiplex PCR. Individualized PCR with the phenol/chloroform DNA extraction protocol detected a larger number of Eimeria species. Molecular diagnosis was found to be practical and precise, and can be used for monitoring and epidemiological studies of Eimeria.
