Related Experiment Video
Updated: May 31, 2026

13:42
Gold Nanoparticle Synthesis
Published on: July 10, 2021
Fast and cost-effective purification of gold nanoparticles in the 20-250 nm size range by continuous density gradient
Dennis Steinigeweg1, Max Schütz, Mohammad Salehi
1Department of Physics, University of Osnabrück, 49069 Osnabrück, Germany.
Small (Weinheim an Der Bergstrasse, Germany)
|June 29, 2011
Abstract:
A multilayer quasi-continuous density gradient centrifugation method for separating 20-250 nm metal colloids with high size resolution while maintaining particle stability is presented. Colloidal mixtures containing monodisperse gold nanospheres and clusters thereof, in particular, gold dimers, are purified with yields up to 94%. The rapid method uses standard laboratory equipment.
More Related Videos
Related Concept Videos
Centrifugation
Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
Subcellular Fractionation
The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
Differential Centrifugation
Differential centrifugation is...

