Sulfonated cyclofructan 6 based stationary phase for hydrophilic interaction chromatography.

Area of Science:

• Analytical Chemistry
• Chromatography
• Separation Science

Background:

• Hydrophilic interaction chromatography (HILIC) is crucial for separating polar compounds.
• Development of novel stationary phases is essential for advancing HILIC capabilities.
• Existing HILIC columns may have limitations in separating a wide range of polar analytes.

Purpose of the Study:

• To synthesize and evaluate a novel stationary phase based on silica-bonded sulfonated cyclofructan 6 (SCF6) for HILIC.
• To assess the separation performance of the SCF6 stationary phase for various polar compounds.
• To compare the performance of the SCF6 phase with commercially available HILIC columns.

Main Methods:

• Synthesis of silica-bonded SCF6 stationary phase.
• Evaluation of separation for diverse polar compounds using HILIC.
• Optimization of mobile phase composition and pH.
• Analysis of analyte retention dependence on temperature via van't Hoff plots.
• Comparison with commercial HILIC columns.

Main Results:

• The SCF6 stationary phase successfully separated a wide array of polar and hydrophilic compounds.
• Separated analytes included beta blockers, xanthines, salicylic acid derivatives, nucleic acid bases, nucleosides, maltooligosaccharides, water-soluble vitamins, and amino acids.
• Separation conditions were optimized by adjusting mobile phase parameters.
• The SCF6 phase demonstrated broad applicability in HILIC mode.

Conclusions:

• The newly synthesized silica-bonded SCF6 stationary phase is effective for HILIC.
• This SCF6 phase offers a versatile platform for the separation of numerous polar and hydrophilic compounds.
• The developed stationary phase shows promise for various analytical applications requiring HILIC separation.