Optimization of the coating procedure for a high-throughput 96-blade solid phase microextraction system coupled with...

Area of Science:

• Analytical Chemistry
• Materials Science

Background:

• Solid Phase Microextraction (SPME) is a widely used technique for sample preparation.
• Traditional SPME methods often face limitations in throughput and extraction efficiency.
• Development of advanced extraction phases and automated systems is crucial for high-throughput analysis.

Purpose of the Study:

• To develop and evaluate a biocompatible C18-polyacrylonitrile (PAN) coating for an automated 96-blade SPME system.
• To optimize the coating preparation method for enhanced stability and reusability.
• To assess the performance of the thin-film SPME system in terms of extraction recovery, reproducibility, and reliability.

Main Methods:

• Preparation of C18-PAN coating using dipping, brush painting, and spraying methods.
• Evaluation of coating stability and reusability.
• Implementation of a robotic autosampler for high-throughput sample preparation (96 samples simultaneously).
• Comparison of extraction recovery with C18-PAN rod fibers.
• Assessment of various factors including reusability, reproducibility, and pH stability.

Main Results:

• The spraying method proved optimal for coating preparation, offering superior stability and reusability.
• The 96-blade SPME system with C18-PAN thin-film coating demonstrated significantly enhanced extraction recovery compared to rod fibers.
• The system exhibited good long-term reusability, reproducibility, and biocompatibility.
• Achieved limits of detection and quantitation in the range of 0.1-0.3 ng/mL and 0.5-1 ng/mL, respectively.

Conclusions:

• The developed C18-PAN 96-blade SPME coating is a robust and efficient tool for high-throughput sample preparation.
• The automated system offers improved analytical performance, including enhanced extraction recovery and reliability.
• This technology holds promise for various applications requiring sensitive and efficient analysis of analytes.