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Real-time RT-PCR for detection of equine influenza and its evaluation using samples from horses infected with

A J Foord1, P Selleck, A Colling

  • 1CSIRO Livestock Industries, Australian Animal Health Laboratory, 5 Portarlington Road (Private Bag 24), Geelong, VIC 3220, Australia.

Australian Veterinary Journal
|June 30, 2011
PubMed
Abstract

No abstract available in PubMed .

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...