Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR activation may...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Azoarene activation for Schmidt-type reaction and mechanistic insights.

Nature communications·2022
Same author

Neutrophil-to-lymphocyte Ratio Predicts the Outcome of Cerebral Venous Thrombosis.

Current neurovascular research·2021
Same author

What do COVID-19 Tweets Reveal about Public Engagement with Nature of Science?

Science & education·2021
Same author

Variable Induction of Pro-Inflammatory Cytokines by Commercial SARS CoV-2 Spike Protein Reagents: Potential Impacts of LPS on In Vitro Modeling and Pathogenic Mechanisms In Vivo.

International journal of molecular sciences·2021
Same author

Assessment of continuous neutrophil CD64 index measurement for diagnosing sepsis and predicting outcome in a Chinese pediatric intensive care unit: a prospective study.

Translational pediatrics·2021
Same author

Genetic basis of maize kernel oil-related traits revealed by high-density SNP markers in a recombinant inbred line population.

BMC plant biology·2021
Same journal

IL-6 and TNFα are associated with depressive symptoms among men in a community-based cohort, with a tentative trend for IL-17A: Findings from the Shika Study in Japan.

Neuro endocrinology letters·2026
Same journal

Information and communication technologies and social media in nursing practice: From the perspective of nurses and patients.

Neuro endocrinology letters·2026
Same journal

Hematological Profiles in Women with Lipedema: Exploratory Analysis of Platelet Distribution Width and Mean Platelet Volume. .

Neuro endocrinology letters·2026
Same journal

The Association Between Handgrip Strength and Type 2 Diabetes Mellitus in Community Dwelling Older Adults in Nanjing, China.

Neuro endocrinology letters·2026
Same journal

Toxic masculinity and depression in men: A schema therapy perspective.

Neuro endocrinology letters·2026
Same journal

Impact of Helicobacter pylori- and Porphyromonas gingivalis-associated metabolic syndrome on local and systemic disorders.

Neuro endocrinology letters·2026
See all related articles

Related Experiment Video

Updated: May 31, 2026

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity
09:45

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity

Published on: January 29, 2018

SUMO negatively regulates BACE expression.

Hui Fang1, Xin Du, Fan-Tao Meng

  • 1School of Life Science, University of Science and Technology of China, Hefei, China. jnzhou@ustc.edu.cn

Neuro Endocrinology Letters
|June 30, 2011
PubMed
Summary
This summary is machine-generated.

Small Ubiquitin-like Modifier (SUMO) proteins negatively regulate BACE gene expression. This regulation involves the transcription factor SP1, impacting APP processing.

More Related Videos

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells
09:40

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells

Published on: November 2, 2017

SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer
08:29

SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

Published on: November 1, 2019

Related Experiment Videos

Last Updated: May 31, 2026

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity
09:45

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity

Published on: January 29, 2018

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells
09:40

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells

Published on: November 2, 2017

SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer
08:29

SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

Published on: November 1, 2019

Area of Science:

  • Molecular Biology
  • Biochemistry

Background:

  • Beta-secretase 1 (BACE1) plays a critical role in amyloid precursor protein (APP) processing.
  • The role of protein modification, specifically sumoylation, in regulating BACE1 expression is not fully understood.

Purpose of the Study:

  • To investigate the effect of SUMO on BACE1 promoter activity and mRNA expression.
  • To elucidate the connection between sumoylation and APP processing via BACE1 regulation.

Main Methods:

  • Reporter gene assays were used to measure BACE1 promoter activity.
  • Real-time RT-PCR was employed to quantify BACE1 mRNA levels.
  • The role of the SP1 transcription factor binding site was assessed by disruption and inhibition.

Main Results:

  • Overexpression of SUMO proteins inhibited BACE1 gene promoter activity.
  • Disruption of the SP1 binding site blocked SUMO-mediated inhibition of promoter activity.
  • SUMO proteins negatively regulated endogenous BACE1 mRNA levels.
  • Inhibition of SP1 activity further reduced BACE1 promoter activity.

Conclusions:

  • SUMO proteins exert a negative regulatory effect on BACE1 expression.
  • The transcription factor SP1 is involved in the SUMO-mediated regulation of BACE1.