Transmission electron microscopy of amyloid fibrils
Sally L Gras1, Lynne J Waddington, Kenneth N Goldie
1Department of Chemical and Biomolecular Engineering, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC, Australia. sgras@unimelb.edu.au
Abstract:
Transmission Electron Microscopy of negatively stained and cryo-prepared specimens allows amyloid fibrils to be visualised at high resolution in a dried or a hydrated state, and is an essential method for characterising the morphology of fibrils and pre-fibrillar species. We outline the key steps involved in the preparation and observation of samples using negative staining and cryo-electron preservation. We also discuss methods to measure fibril characteristics, such as fibril width, from electron micrographs.
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