Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Doppler Effect - II01:05

Doppler Effect - II

The Doppler effect has several practical, real-world applications. For instance, meteorologists use Doppler radars to interpret weather events based on the Doppler effect. Typically, a transmitter emits radio waves at a specific frequency toward the sky from a weather station. The radio waves bounce off the clouds and precipitation and travel back to the weather station. The radio frequency of the waves reflected back to the station appears to decrease if the clouds or precipitation are moving...
Doppler Effect - I00:56

Doppler Effect - I

The Doppler effect and Doppler shift were named after the Austrian physicist and mathematician Christian Johann Doppler in 1842, who conducted experiments with both moving sources and moving observers. Consider an observer standing on a street corner, observing an ambulance with a siren sound passing by at a constant speed. The observer experiences two characteristic changes in the sound of the siren. Initially, the sound increases in loudness as the ambulance approaches and decreases in...
Computed Tomography01:10

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pediatric blood pressure vulnerability to meteorological hazards: Lagged and nonlinear effects of temperature-humidity exposure in urban China.

Environmental research·2026
Same author

Impact of the β correction factor on the accuracy of speckle contrast imaging measurements.

Biomedical optics express·2026
Same author

Lightweight Head-Mounted Optical Coherence Tomography Scope for Longitudinal, Intact-Skull Imaging of Ischemic Stroke in Freely Behaving Mouse.

ACS photonics·2026
Same author

Optical and acoustic scattering in cutaneous neurofibromas: Implications for early detection.

The Journal of investigative dermatology·2026
Same author

Comparative Evaluation of R134a and HFO-1234ze Cryogen Spray Cooling Using a Mouse Model With Controllable Epidermal Pigmentation.

Lasers in surgery and medicine·2026
Same author

Full-Density TiB<sub>2</sub> Cathodes for Energy-Efficient Aluminum Electrolysis via Oriented Assembly in Molten Salts.

ACS nano·2026
Same journal

Long-term stabilization of intensity-difference squeezing from four-wave mixing in rubidium vapor.

Optics express·2026
Same journal

Robust 3D topography measurement of large-range high-aspect-ratio structures based on dual-domain statistical filtering in SD-OCT.

Optics express·2026
Same journal

Broadband transmissive terahertz metasurface for simultaneous quad-mode OAM multiplexing.

Optics express·2026
Same journal

Leveraging two-dimensional materials for high-sensitivity optical sensors: quasi-bound states in the continuum within hybrid metasurfaces.

Optics express·2026
Same journal

Resolution investigation for dual-spherical-wave optical scanning holographic microscopy: methods and performance.

Optics express·2026
Same journal

Robustness of parallel subnetwork-filtered diffractive deep neural networks.

Optics express·2026
See all related articles

Related Experiment Video

Updated: May 31, 2026

Doppler Optical Coherence Tomography of Retinal Circulation
10:46

Doppler Optical Coherence Tomography of Retinal Circulation

Published on: September 18, 2012

Intensity-based modified Doppler variance algorithm: application to phase instable and phase stable optical coherence

Gangjun Liu1, Lidek Chou, Wangcun Jia

  • 1Beckman Laser Institute, University of California, Irvine, Irvine, California 92612, USA.

Optics Express
|July 1, 2011
PubMed
Summary
This summary is machine-generated.

A new modified Doppler variance algorithm improves in-vivo blood flow imaging by reducing artifacts caused by phase instability in optical coherence tomography (OCT) systems.

More Related Videos

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Related Experiment Videos

Last Updated: May 31, 2026

Doppler Optical Coherence Tomography of Retinal Circulation
10:46

Doppler Optical Coherence Tomography of Retinal Circulation

Published on: September 18, 2012

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Area of Science:

  • Biomedical Optics
  • Medical Imaging
  • Optical Coherence Tomography

Background:

  • Traditional phase-resolved Doppler methods excel in blood flow imaging but require high system phase stability.
  • Phase instability degrades the performance of conventional Doppler imaging techniques, limiting their clinical applicability.
  • Artifacts from phase instability hinder accurate visualization of microvasculature in optical coherence tomography (OCT).

Purpose of the Study:

  • To develop and validate a modified Doppler variance algorithm robust to phase instability for enhanced in-vivo blood flow imaging.
  • To compare the proposed algorithm's performance against traditional phase-resolved Doppler variance and color Doppler methods.
  • To demonstrate the algorithm's efficacy in phase-stable and phase-unstable optical coherence tomography (OCT) systems.

Main Methods:

  • A modified Doppler variance algorithm based on intensity or amplitude values was developed.
  • The proposed algorithm was evaluated against traditional methods in both phase-stable and phase-unstable OCT systems.
  • In-vivo imaging was performed using spectrometer-based Fourier domain OCT and a microelectromechanical systems (MEMS) based swept source OCT (SSOCT) system.

Main Results:

  • The modified algorithm successfully generated artifact-free images in phase-unstable conditions, unlike traditional methods.
  • In-vivo imaging of hamster skin and human skin vasculature demonstrated the algorithm's effectiveness in phase-unstable scenarios.
  • Comparable performance to phase-resolved methods was observed in phase-stable conditions, including detailed imaging of the human choroidal vasculature.

Conclusions:

  • The proposed intensity/amplitude-based Doppler variance algorithm offers a robust solution for in-vivo blood flow imaging, particularly in phase-unstable OCT systems.
  • This method overcomes limitations of traditional Doppler techniques, enabling clearer visualization of microvasculature without phase instability artifacts.
  • The algorithm shows significant potential for improving diagnostic capabilities in OCT-based vascular imaging across various physiological conditions.