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Updated: May 31, 2026

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis
Published on: June 24, 2018
Microstructure and chemical analysis of blood-contaminated mineral trioxide aggregate
M H Nekoofar1, T E Davies, D Stone
1Endodontology Research Group, School of Dentistry, Cardiff University, Cardiff, UK. nekoofarmh@cardiff.ac.uk
Aim:
To test the hypothesis that blood contamination has a detrimental effect on the chemical properties of Mineral trioxide aggregate (MTA).
Methodology:
The effects of whole, fresh human blood on the microstructure and elemental chemistry of MTA were evaluated using scanning electron microscopy and energy-dispersive X-ray analysis, respectively. The phase compositions of contaminated and uncontaminated MTA were also analysed using X-ray diffraction analysis.
Results:
The hydration state of specimens partially mixed with blood were more complete than those mixed entirely with blood and less than those entirely mixed with water. Acicular crystals, characteristic of ettringite, were abundant in specimens mixed entirely with water and absent from specimens mixed partially or entirely with blood. Calcium hydroxide crystals were absent in specimens contaminated entirely with blood and the unhydrated MTA powder, but present in the other groups.
Conclusion:
Mixing MTA with blood resulted in the lack of formation of the crystalline calcium hydroxide in the early stage of the hydration process.
Insights
Blood contamination negatively impacts Mineral Trioxide Aggregate (MTA) by hindering the formation of calcium hydroxide during early hydration. This affects MTA's chemical properties and microstructure.
Area of Science:
- Dental Materials Science
- Biomaterials Chemistry
Background:
- Mineral Trioxide Aggregate (MTA) is a widely used dental material.
- Understanding factors affecting MTA's properties is crucial for clinical success.
Purpose of the Study:
- To investigate the detrimental effects of blood contamination on the chemical properties of Mineral Trioxide Aggregate (MTA).
Main Methods:
- Scanning electron microscopy (SEM) and energy-dispersive X-ray analysis (EDX) were used to assess microstructure and elemental chemistry.
- X-ray diffraction analysis (XRD) evaluated phase compositions of contaminated and uncontaminated MTA.
Main Results:
- Blood contamination altered the hydration state of MTA specimens.
- Ettringite crystal formation was absent in blood-contaminated MTA.
- Calcium hydroxide formation was inhibited in MTA mixed with blood.
Conclusions:
- Blood contamination significantly interferes with the early hydration process of MTA.
- The absence of calcium hydroxide formation indicates altered chemical properties of contaminated MTA.
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