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Cloning and chromosomal assignment of a widely expressed human receptor-like protein-tyrosine phosphatase
F R Jirik1, N M Janzen, I G Melhado
1Biomedical Research Center, University of British Columbia, Vancouver, Canada.
Abstract:
Insight into the regulation of the actions of the protein-tyrosine kinases will be greatly facilitated by the full characterization of the family of protein-tyrosine phosphatases. A search for novel phosphatases resulted in the isolation of a cDNA, termed HLPR, encoding a member of the family of human receptor-like protein-tyrosine phosphatases: its cDNA sequence predicts a protein of 793 amino acids (unglycosylated Mr 87,500) and includes a 121 residue extracellular domain, a single transmembrane segment, and and two tandem intra-cytoplasmic catalytic domains. The HLPR genes is located on human chromosome 20, and the protein it encodes likely plays a fundamental role in the physiology of all cells as its expression appears to be ubiquitous.
Insights
Researchers identified a novel human receptor-like protein-tyrosine phosphatase, named HLPR. This ubiquitous protein, found on chromosome 20, has a structure suggesting a fundamental role in cell physiology.
Area of Science:
- Molecular Biology
- Cell Biology
- Biochemistry
Background:
- Protein-tyrosine kinases are crucial regulators of cellular processes.
- Understanding protein-tyrosine phosphatases is essential for elucidating kinase regulation.
- A comprehensive characterization of phosphatases is needed.
Purpose of the Study:
- To identify and characterize novel protein-tyrosine phosphatases.
- To investigate the structure and potential function of newly discovered phosphatases.
Main Methods:
- Screening for novel phosphatases.
- Isolation and characterization of a complementary DNA (cDNA) clone.
- Sequence analysis of the cDNA to predict protein structure.
- Gene localization using human chromosome mapping.
Main Results:
- A novel cDNA, termed HLPR, was isolated.
- HLPR encodes a human receptor-like protein-tyrosine phosphatase.
- The predicted protein has 793 amino acids with extracellular, transmembrane, and intracellular catalytic domains.
- The HLPR gene is located on human chromosome 20.
- HLPR expression is ubiquitous across cells.
Conclusions:
- HLPR represents a new member of the human receptor-like protein-tyrosine phosphatase family.
- The structural features of HLPR suggest a significant role in cellular physiology.
- Ubiquitous expression indicates a fundamental function in diverse cell types.