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Updated: May 31, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
Epidemic population structure of extraintestinal pathogenic Escherichia coli determined by single nucleotide
Natalia Fernández-Romero1, María Pilar Romero-Gómez, María Rosa Gómez-Gil
1Servicio de Microbiología, Hospital Universitario La Paz, IdiPAZ, Paseo de La Castellana, Madrid, Spain.
Abstract:
We have developed an MLST-based scheme for typing Escherichia coli isolates using pyrosequencing of single nucleotide polymorphic positions (SNP). The SNP sequences are converted into allelic patterns and analyzed using the same approach used for MLST analyses. We have tested the method in two unselected collections of clinical isolates of E. coli obtained from blood and urine cultures. The two collections had a similar structure, 25% of the profiles (representing 68% of the isolates) were common to both, and 62% of the profiles (nearly 20% of the isolates) were unique. The four major profiles accounted for 44% of the isolates, and among these the most frequent one was related to the pandemic ST131 clone. The method is easy to implement and might be useful for typing large microbial collections.
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