Related Experiment Video
Updated: May 31, 2026
08:54
An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells
Published on: July 20, 2014
Crosstalk between activated and inactivated c-Src in hepatocellular carcinoma
Mei-Lin Chen1, Chee-Yin Chai, Kun-Tu Yeh
1Department of Pathology, Changhua Christian Hospital, Taiwan.
Disease Markers
|July 5, 2011
Summary
Active c-Src (p-Tyr416-c-Src) is a potential prognostic marker for hepatocellular carcinoma (HCC) survival. Its expression, along with non-phosphorylated c-Src (non-p-Tyr527-c-Src), offers insights into HCC patient outcomes.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- c-Src activity is regulated by tyrosine phosphorylation at Tyr416 and Tyr527 sites.
- The clinical significance of these phosphorylation sites in human cancers, particularly hepatocellular carcinoma (HCC), remains unclear.
Purpose of the Study:
- To investigate the role of c-Src phosphorylation at Tyr416 and Tyr527 in hepatocellular carcinoma (HCC).
- To determine if alterations and crosstalk between these sites contribute to HCC development and patient prognosis.
Main Methods:
- Immunohistochemistry was used to analyze the expression patterns of phosphorylated and non-phosphorylated c-Src at Tyr416 and Tyr527 in 85 HCC patient specimens.
- Statistical correlations were performed between these expression patterns and clinicopathological characteristics, including tumor grade, stage, vascular invasion, and patient survival rates.
Main Results:
- Active c-Src (p-Tyr416-c-Src) correlated positively with tumor grade and inversely with vascular invasion.
- Non-phosphorylated c-Src (non-p-Tyr416-c-Src) correlated with tumor stage and grade.
- Inactive c-Src (p-Tyr527-c-Src) levels differed between male and HCV-infected patients.
- Increased p-Tyr416-c-Src and decreased non-p-Tyr527-c-Src were associated with poor survival.
- Low p-Tyr416-c-Src and non-p-Tyr527-c-Src expression predicted prolonged survival.
- p-Tyr416-c-Src expression was an independent predictor of HCC patient survival.
Conclusions:
- Active c-Src (p-Tyr416-c-Src) may serve as an independent prognostic marker for HCC patient survival.
- The interplay between different c-Src phosphorylation states warrants further investigation in HCC development.
Related Concept Videos
02:34Inhibition of Cdk Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
01:19Interactions Between Signaling Pathways
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
03:03mTOR Signaling and Cancer Progression
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
02:38The Ras Gene
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a superfamily...
Ras is a superfamily...
01:07MAPK Signaling Cascades
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
01:06Induced Pluripotent Stem Cells
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Somatic cells are...