Related Experiment Video
Updated: May 31, 2026
09:32
Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
Published on: October 14, 2022
Highlighting chromosome loops in DNA-picked chromatin (DPC)
Ciro Abbondanza1, Caterina De Rosa, Maria Neve Ombra
1Dipartimento di Patologia Generale, Seconda Università degli Studi di Napoli; Naples, Italy.
Epigenetics
|July 5, 2011
Summary
This study reveals that 17β-estradiol-induced transcription involves looping between gene promoter and termination regions. This finding offers a new method for analyzing gene expression correlations and shared regulatory sites.
Area of Science:
- Molecular Biology
- Genetics
- Epigenetics
Background:
- Cell-type specific gene expression is regulated by dynamic intra- and inter-chromosomal interactions.
- Understanding functional correlations between distant DNA sites governing transcription is crucial.
Purpose of the Study:
- To investigate the role of DNA looping in hormone-responsive gene transcription.
- To propose an experimental approach for analyzing gene-regulatory site correlations.
Main Methods:
- Utilized an experimental approach to study 17β-estradiol-induced transcription.
- Focused on the formation of loops between promoter and termination regions of hormone-responsive genes.
Main Results:
- Demonstrated that 17β-estradiol-induced transcription is associated with the formation of DNA loops.
- Identified loops connecting promoter and termination regions of hormone-responsive genes.
Conclusions:
- The formation of DNA loops is a key mechanism in hormone-induced gene expression.
- The proposed strategy can be used with automated techniques for extensive analysis of shared regulatory sites.
Related Concept Videos
02:04Polytene Chromosomes
Polytene chromosomes are giant interphase chromosomes with several DNA strands placed side by side. They were discovered in the year 1881 by Balbiani in salivary glands, intestine, muscles, malpighian tubules, and hypoderm of larvae Chironomus plumosus. Hence, these are also called "Salivary gland chromosomes." These are found in insects of the order Diptera and Collembola; in certain organs of mammals; and synergids, antipodes of flowering plants. Polytene chromosomes are also regularly...
01:51Lampbrush Chromosomes
In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops resemble the...
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops resemble the...
02:36Chromatin Immunoprecipitation- ChIP
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
01:17Karyotyping
Overview
02:05Duplication of Chromatin Structure
The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
02:38Heterochromatin
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...