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Evaluation of countermeasures for reduction of mouse airborne allergens
M Sakaguchi1, S Inouye, H Miyazawa
1Department of Microbiology, Kyorin University, Tokyo, Japan.
Abstract:
Three kinds of countermeasures for reduction of mouse airborne allergens were evaluated with use of an air sampler and immunochemical methods. Mouse cages and the sampler were placed inside a flexible-film isolator, and concentrations of mouse major allergens in the air were measured. The levels of the airborne allergens, prealbumin and albumin, generated by 10 males, were 3,050 and 492 pg/m3, respectively. Those by 10 females were lower, 317 and 270 pg/m3, respectively. When mouse cages were covered with a filter cap, the airborne allergens inside the isolator were decreased by 90%. When corncob was used as bedding in place of wood shavings, the airborne allergens were decreased by 57 and 77%, respectively. Therefore, for reduction of mouse airborne allergens, we recommend using female mice, covering the cages with filter caps, and using corncob bedding.
Insights
Reducing mouse airborne allergens is achievable. Using female mice, filter caps on cages, and corncob bedding significantly lowers allergen levels, improving air quality for researchers and sensitive individuals.
Area of Science:
- Allergen research
- Laboratory animal science
- Environmental health
Background:
- Mouse allergens, specifically prealbumin and albumin, are significant airborne contaminants in laboratory settings.
- Exposure to these allergens can cause respiratory issues and allergic reactions in sensitized individuals.
- Quantifying airborne allergen levels is crucial for developing effective control strategies.
Purpose of the Study:
- To evaluate the efficacy of three countermeasures for reducing mouse airborne allergens.
- To compare allergen levels produced by male and female mice.
- To provide recommendations for minimizing airborne allergen exposure in research facilities.
Main Methods:
- Air sampling and immunochemical methods were employed within a flexible-film isolator.
- Concentrations of prealbumin and albumin were measured in the air.
- The impact of filter caps on cages and corncob bedding versus wood shavings was assessed.
Main Results:
- Airborne allergen levels were significantly higher in air samples from male mice compared to female mice.
- Covering mouse cages with filter caps reduced airborne allergens by 90%.
- Using corncob bedding instead of wood shavings decreased airborne allergens by 57% and 77%.
Conclusions:
- Female mice generate lower levels of airborne allergens than male mice.
- Filter caps on cages are highly effective in reducing airborne allergens.
- Corncob bedding offers a viable alternative to wood shavings for allergen reduction.