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Updated: May 31, 2026
09:12
A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection
Published on: June 15, 2018
An ultrasensitive immunosensor array for determination of staphylococcal enterotoxin B.
Xiaoming Zhang1, Fei Liu, Ruifang Yan
1Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, China.
Talanta
|July 6, 2011
Summary
A new fluorescence immunoassay accurately detects low levels of staphylococcal enterotoxin B (SEB), a heat-resistant toxin and bioterrorism threat. This method is crucial for food safety and public health monitoring.
Area of Science:
- Food Science
- Immunology
- Biotechnology
Background:
- Staphylococcal enterotoxin B (SEB) is a potent, heat-resistant gastrointestinal toxin.
- SEB poses a significant bioterrorism risk, necessitating accurate detection methods.
- Measuring low SEB concentrations in food and other samples is critical for safety.
Purpose of the Study:
- To develop a highly sensitive and stable sandwich fluorescence immunoassay for SEB detection.
- To utilize a novel detection platform for enhanced sensitivity.
Main Methods:
- A sandwich fluorescence immunoassay was developed using monoclonal antibodies against SEB.
- Glass slides served as the base for the immunologic reaction.
- Functionalized fluorescent core-shell silica nanoparticles were employed as labels, detected by a laser-induced fluorescence millimeter sensor array.
Main Results:
- The assay demonstrated a linear relationship between fluorescence intensity and SEB concentration from 50 pg/mL to 5 ng/mL.
- A detection limit of 20 pg/mL (absolute detection limit of 0.02 pg) was achieved.
- The relative standard deviation for parallel measurements was 9.2%.
Conclusions:
- A sensitive and stable fluorescence immunoassay for SEB detection has been successfully developed.
- The assay is suitable for accurate quantification of SEB at very low levels.
- This method offers a valuable tool for food safety and bioterrorism surveillance.
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