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Updated: May 31, 2026

An Assay for Quantifying Protein-RNA Binding in Bacteria
Published on: June 12, 2019
Structure-activity studies of RNA-binding oxazolidinone derivatives
Iwona Maciagiewicz1, Shu Zhou, Stephen C Bergmeier
1Department of Chemistry and Biochemistry, Ohio University, Athens, OH 45701, USA.
Abstract:
The structure-activity relationship of a series of oxazolidinones binding to T-box riboswitch antiterminator RNA has been investigated. Oxazolidinones differentially substituted at C-5 were prepared and the ligand-induced fluorescence resonance energy transfer (FRET) changes in FRET-labeled antiterminator model RNA were assayed. Both qualitative and quantitative analysis of the structure-activity relationship indicate that hydrogen bonding and hydrophobic properties play a significant role in ligand binding.
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