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Over-expression of chloroperoxidase in Caldariomyces fumago
Markus Buchhaupt1, Kristina Ehrich, Sonja Hüttmann
1DECHEMA eV, Biochemical Engineering, Frankfurt am Main, Germany. buchhaupt@dechema.de
Biotechnology Letters
|July 8, 2011
Abstract:
The filamentous fungus Caldariomyces fumago secretes a chloroperoxidase (CPO). To increase its production, we integrated a CPO-expression cassette into the non-transcribed spacer regions of the rDNA in C. fumago. One strain was obtained that had twice the CPO activity when grown in shake-flask and bioreactor compared to the wild-type. The highest CPO activity from the bioreactor cultivation was 3,236 U ml(-1). This is the highest value reported so far.
