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Updated: May 31, 2026

Use of a Linear Accelerator for Conducting In Vitro Radiobiology Experiments
Published on: May 26, 2019
Dependence of cell survival on instantaneous dose rate of a linear accelerator
Brita Singers Sørensen1, Anne Vestergaard, Jens Overgaard
1Department of Experimental Clinical Oncology, Aarhus University Hospital, Denmark. bsin@oncology.dk
Background And Purpose:
With the development of flattening filter-free linear accelerators for radiotherapy, the instantaneous dose rate has increased by approximately a factor 4. This leads to the question of whether there may be a radiobiological consequence. The present study investigates the radiobiological effect of this high instantaneous dose rate on two cell lines.
Materials And Methods:
A Varian Trilogy TX linear accelerator was used to directly compare the effect of three different dose rates (5.01, 9.99 and 29.91 Gy/min, with instantaneous dose rates in the pulse of 56.5, 112.8 and 338 Gy/s) on clonogenic survival. V79 and FaDu(DD) cells were irradiated with doses in a range from 1 to 10 Gy in order to obtain dose response curves.
Results:
For both cell lines, there is not observed any effect of the instantaneous dose rate on cell survival.
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