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Updated: May 31, 2026

Bimolecular Fluorescence Complementation
Published on: April 15, 2011
Improvement of a Venus-based bimolecular fluorescence complementation assay to visualize bFos-bJun interaction in
Chika Nakagawa1, Kazuto Inahata, Shigenori Nishimura
1Laboratory of Molecular Biology and Cell Informatics, Division of Bioscience and Informatics, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, Japan. cnakagawa@bioinfo.osakafu-u.ac.jp
Abstract:
Bimolecular fluorescence complementation (BiFC) assay makes it possible to visualize protein-protein interactions in living cells. In this assay, Venus, a bright-yellow variant of green fluorescent protein, is known to produce fluorescent backgrounds due to non-specific interactions. In this study we found that the V150A mutation increased by 8.6-fold the signal-to-noise ratio in the BiFC assay of bFos-bJun interaction.
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