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Preparation of 1° Amines: Gabriel Synthesis

Preparation of 1° Amines: Gabriel Synthesis

Direct alkylation is not a suitable method for synthesizing amines because it produces polyalkylated products. Gabriel synthesis is the most preferred method to exclusively make primary amines. The method uses phthalimide, which contains a protected form of nitrogen that participates in alkylation only once to predominantly give primary amines.
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Related Experiment Video

Updated: May 31, 2026

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues

Published on: February 17, 2017

A convenient purification method for pyridylamino monosaccharides.

Shunji Natsuka¹

¹Department of Biology, Faculty of Science, Niigata University, Japan. natsuka@bio.sc.niigata-u.ac.jp

Bioscience, Biotechnology, and Biochemistry

|July 9, 2011

Summary

Purifying pyridylamino monosaccharides is now easier. A new spin column method using boronate-conjugated resin effectively removes interfering by-products for accurate sugar analysis.

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Published on: July 26, 2018

Area of Science:

Analytical Chemistry
Biochemistry

Background:

Pyridylamino derivatization is a common method for sugar microanalysis.
Interference from reaction by-products and sample-derived fluorescent substances can compromise the accuracy of pyridylamino sugar detection, particularly in monosaccharide analysis.

Purpose of the Study:

To develop a convenient and effective purification method for pyridylamino monosaccharides.
To address the issue of interference in the analysis of pyridylamino sugars.

Main Methods:

Development of a purification technique utilizing a spin column packed with boronate-conjugated resin.
Application of the spin column for the isolation of pyridylamino monosaccharides.

Main Results:

The developed method successfully removes interfering substances, including reaction by-products and sample-derived fluorescent compounds.
The purification procedure is convenient and enhances the accuracy of monosaccharide analysis.

Conclusions:

The spin column method with boronate-conjugated resin provides an efficient means to purify pyridylamino monosaccharides.
This technique significantly improves the reliability of sugar microanalysis by mitigating interference issues.