Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhancing Digital Microfluidics: A Comprehensive Investigation into the Performance of Slippery Liquid-Infused Porous Surfaces.

ACS applied materials & interfaces·2025
Same author

Hybrid SiO<sub>2</sub>/Si pillar-based optomechanical crystals for on-chip photonic integration.

Nanophotonics (Berlin, Germany)·2025
Same author

Antifouling slippery liquid infused porous surface for surfactant-free PCR on digital microfluidics platform.

Talanta·2024
Same author

ZIF-8-Based Surface Plasmon Resonance and Fabry-Pérot Sensors for Volatile Organic Compounds.

Sensors (Basel, Switzerland)·2024
Same author

Biosynthesis enhancement of tropodithietic acid (TDA) antibacterial compound through biofilm formation by marine bacteria <i>Phaeobacter inhibens</i> on micro-structured polymer surfaces.

RSC advances·2023
Same author

Using Impedance Flow Cytometry for Rapid Viability Classification of Heat-Treated Bacteria.

ACS omega·2023

Related Experiment Video

Updated: May 31, 2026

Designing Microfluidic Devices for Studying Cellular Responses Under Single or Coexisting Chemical/Electrical/Shear Stress Stimuli
10:35

Designing Microfluidic Devices for Studying Cellular Responses Under Single or Coexisting Chemical/Electrical/Shear Stress Stimuli

Published on: August 13, 2016

Microfluidic device to study cell transmigration under physiological shear stress conditions.

Dorota Kwasny1, Katrine Kiilerich-Pedersen, Jacob Moresco

  • 1Department of Micro- and Nanotechnology, Technical University of Denmark, Ørsteds Plads, 2800 Kgs. Lyngby, Denmark. dorota.kwasny@nanotech.dtu.dk

Biomedical Microdevices
|July 9, 2011
PubMed
Summary

This study introduces a novel microfluidic assay for studying cell transmigration under conditions that mimic blood vessels. This innovative tool enhances drug development by providing a more realistic in vitro model for cell migration research.

More Related Videos

A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation
12:55

A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation

Published on: December 9, 2021

A Novel Three-dimensional Flow Chamber Device to Study Chemokine-directed Extravasation of Cells Circulating under Physiological Flow Conditions
10:56

A Novel Three-dimensional Flow Chamber Device to Study Chemokine-directed Extravasation of Cells Circulating under Physiological Flow Conditions

Published on: July 15, 2013

Related Experiment Videos

Last Updated: May 31, 2026

Designing Microfluidic Devices for Studying Cellular Responses Under Single or Coexisting Chemical/Electrical/Shear Stress Stimuli
10:35

Designing Microfluidic Devices for Studying Cellular Responses Under Single or Coexisting Chemical/Electrical/Shear Stress Stimuli

Published on: August 13, 2016

A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation
12:55

A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation

Published on: December 9, 2021

A Novel Three-dimensional Flow Chamber Device to Study Chemokine-directed Extravasation of Cells Circulating under Physiological Flow Conditions
10:56

A Novel Three-dimensional Flow Chamber Device to Study Chemokine-directed Extravasation of Cells Circulating under Physiological Flow Conditions

Published on: July 15, 2013

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Pharmacology

Background:

  • Traditional cell transmigration assays (Boyden chamber, shear flow chamber) do not accurately mimic natural cell migration.
  • In vitro cell migration studies are crucial for developing new drug therapies.

Purpose of the Study:

  • To develop a novel microfluidic assay for in vitro cell transmigration that better mimics physiological conditions.
  • To create an inexpensive, easy-to-fabricate, and disposable device for cell migration research.

Main Methods:

  • The microfluidic device integrates principles of Boyden and shear flow chambers for transmigration under flow.
  • A 3D cell migration environment is created using cell adhesion proteins (fibronectin, VCAM-1).
  • Jurkat cells and chemokine-induced lymphocytes were used to test migration towards serum and chemoattractants under continuous flow.

Main Results:

  • The assay successfully mimicked physiological shear flow conditions found in blood vessels.
  • A continuous flow system maintained stable concentration gradients of chemoattractants over time and space.
  • The multistep cell transmigration process was effectively observed using the developed microfluidic assay.

Conclusions:

  • The novel microfluidic assay provides a more physiologically relevant platform for studying cell transmigration.
  • This inexpensive and disposable device has significant potential for basic research and drug development.
  • The assay enables detailed observation of cell migration dynamics under controlled flow conditions.