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Related Concept Videos

Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
The Proteasome02:18

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
The Proteasome01:13

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3 (ubiquitin...

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Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae
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Published on: February 16, 2015

Cdc48: a power machine in protein degradation.

Alexandra Stolz1, Wolfgang Hilt, Alexander Buchberger

  • 1Institute for Biochemistry, University of Stuttgart, Pfaffenwaldring 55, 70569 Stuttgart, Germany.

Trends in Biochemical Sciences
|July 12, 2011
PubMed
Summary

The Cdc48 (also known as p97) protein complex is a vital ATP-driven machine in eukaryotic cells, crucial for numerous cellular processes including protein degradation via the proteasome or lysosome.

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Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae
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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Cdc48/p97 is an essential ATP-driven molecular machine in eukaryotic cells.
  • It plays critical roles in diverse cellular processes such as cell cycle, membrane fusion, and gene regulation.
  • Its function is well-characterized in endoplasmic reticulum-associated protein degradation (ERAD).

Purpose of the Study:

  • To review the general characteristics of the Cdc48/p97 complex.
  • To summarize recent findings on Cdc48's molecular functions in various cellular pathways.
  • To highlight Cdc48's role in proteolysis-connected degradation pathways.

Main Methods:

  • Literature review of recent research on Cdc48/p97.
  • Analysis of molecular mechanisms underlying Cdc48 function.
  • Synthesis of findings related to proteasomal and lysosomal degradation pathways.

Main Results:

  • Cdc48/p97 is involved in a wide array of cellular functions beyond ERAD.
  • Recent studies reveal its intricate roles in processes culminating in proteolysis.
  • These degradation pathways include both the ubiquitin-proteasome system and autophagocytosis.

Conclusions:

  • Cdc48/p97 is a versatile molecular machine with broad cellular functions.
  • Its role in orchestrating protein degradation through distinct pathways is a key focus.
  • Understanding Cdc48/p97 is crucial for comprehending cellular homeostasis and disease.